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化学学报
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化学学报 ›› 2011, Vol. 69 ›› Issue (04): 411-417. 上一篇    下一篇

研究论文

普罗帕酮与曙红Y的双荧光猝灭反应及其分析应用研究

田伦富，胡小莉，刘忠芳，孔玲，刘绍璞*   

  1. (发光与实时分析教育部重点实验室 西南大学化学化工学院 重庆 400715)
  • 投稿日期:2010-09-03 修回日期:2010-10-10 发布日期:2010-10-25
  • 通讯作者: 刘绍璞 E-mail:liusp@swu.edu.cn
  • 基金资助:

    生物大分子间相互作用和分子识别的共振瑞利散射和共振非线性散射光谱研究

Mutual Fluorescence Quenching between Propafenone and Eosin Y and Its Analytical Application

TIAN Lun-Fu, HU Xiao-Li, LIU Zhong-Fang, KONG Ling, LIU Shao-Pu   

  1. (Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715)
  • Received:2010-09-03 Revised:2010-10-10 Published:2010-10-25
  • Contact: Shaopu Liu E-mail:liusp@swu.edu.cn

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被引次数

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曙红Y(EY)是一种荧光染料, 它具有强的荧光, 而普罗帕酮(PPF)也是一种荧光物质. 在pH 2.5～3.8的酸性溶液中, EY的最大发射波长(λem)位于549 nm, PPF的λem则在419 nm处, 两者的荧光光谱相距较远而能很好地分开. 当PPF与EY反应形成复合物时, 观察到两者的荧光均发生猝灭, 因此这是一种双荧光猝灭的反应体系. 当用EY作探针测定PPF时, 检出限(3σ)为9.4 ng/mL|反之当用PPF作探针测定EY时, 检出限为259.7 ng/mL, 前者具有很高的灵敏度, 后者灵敏度较低, 因此方法更适合于对痕量PPF的定量测定. 研究了荧光猝灭反应的适宜条件、影响因素和分析化学特性, 并探讨了反应机理和复合物的组成和结构, 结果表明两者通过静电引力、疏水作用、芳基堆积作用和氢键作用而形成1∶1的复合物. 荧光猝灭是一种静态猝灭过程. 因此以EY作探针荧光猝灭法测定PPF为例, 考察了方法的分析应用.

关键词: 普罗帕酮, 曙红Y, 双荧光猝灭反应

Eosin Y (EY), a dye, can emit strong fluorescence, while propafenone (PPF) is also a fluorescent species. The wavelength of maximum emission of them in solution of pH 2.5～3.8 is at 549 nm (EY) and 419 nm (PPF), respectively. Their respective spectra are far from each other so that they can be separated. The fluorescence quenching of both EY and PPF could be observed when they reacted to form complexes, which indicated that this was a mutual fluorescence quenching system. When EY was used as a probe for the determination of PPF, the detection limit (3σ) was 9.4 ng/mL|contrariwise, when PPF was used as a probe, the detection limit for EY (3σ) was 259.7 ng/mL. The former had higher sensitivity so that it was more suitable for the determination of trace PPF. The optimum reaction conditions, influencing factors and analytical properties had been studied. In addition, the discussion on the reaction mechanism, composition and structure of the complex suggests that EY reacted with PPF to form a 1∶1 complex via electrostatic forces, hydrophobic interaction, aromatic stacking interaction and hydrogen bonding. Meanwhile, this fluorescence quenching was a static quenching process. The method was satisfactorily applied to the determination of PPF in tablets and human serum.

Key words: propafenone, eosin Y, mutual fluorescence quenching