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化学学报
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化学学报 ›› 2011, Vol. 69 ›› Issue (11): 1368-1374. 上一篇    下一篇

研究论文

“甘草附子汤”和“术附汤”肠内生物转化的电喷雾质谱研究

王曦烨1,2，皮子凤1，宋凤瑞1，刘志强1，刘淑莹*,1   

  1. (1中国科学院长春应用化学研究所 长春 130022)
    (2中国科学院研究生院 北京 100039)
  • 投稿日期:2010-08-31 修回日期:2010-11-10 发布日期:2011-01-27
  • 通讯作者: 刘淑莹 E-mail:mslab@ciac.jl.cn
  • 基金资助:

    973中医理论专项基金(No.2006CB504706)

Studies on the Biotransformation of Licorice and Aconite Accessory Root Decoction and Atractylodes Macrocephala and Aconite Accessory Root Decoction under Rat Intestinal Bacteria

Wang Xiye1,2 Pi Zifeng1 Song Fengrui1 Liu Zhiqiang1 Liu Shuying*,1   

  1. (1 Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022)
    (2 Graduate School of the Chinese Academy of Science, Beijing 100039)
  • Received:2010-08-31 Revised:2010-11-10 Published:2011-01-27

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1155

被引次数

5

以电喷雾质谱法作为研究方法, 以内标化合物为切入点, 对复方中双酯型、单酯型及脂型生物碱的生物转化进行了深入研究, 建立了电喷雾质谱对代谢前后生物碱成分的半定量分析方法. 分析了中药复方甘草附子汤和术附汤经大鼠肠内菌群代谢主要生物碱的含量变化, 研究结果表明, 配伍中药甘草和白术可以有效地降低共煎液中双酯型生物碱含量, 在代谢过程中, 大鼠肠内菌群能够进一步将复方中双酯型生物碱转化为脂型生物碱, 从而达到中药配伍的减毒增效目的.

关键词: 甘草附子汤, 术附汤, 肠内菌代谢, 电喷雾质谱

The biotransformation of diester-diterpenoid alkaloids, monoester-diterpenoid alkaloids and lipo-alkaloids in compound recipe was studied by electrospray ionization tandem mass spectrometry and a method of semiquantitative ESI-MS was established for analysis of aconitum alkaloids before and after metabolism. The content changes in the process of metabolism of aconitum alkaloids in Licorice and Aconite Accessory Root Decoction and Atractylodes Macrocephala and Aconite Accessory Root Decoction was analyzed under rat intestinal bacteria and the results showed that Licorice and Atractylodes Macrocephala can effectively reduce the content of diester-diterpenoid alkaloids after the compatibility and the rat intestinal bacteria can more reduce the toxicity of diester-diterpenoid alkaloids and increase the content of lipo-alkaloids. The mechanism of compatibility and metabolism of traditional Chinese medicine was proved using the chemical method.

Key words: Licorice and Aconite Accessory Root Decoction, Atractylodes Macrocephala and Aconite Accessory Root Decoction, metabolism, electrospray ionization mass spectrometry