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化学学报
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化学学报 ›› 2011, Vol. 69 ›› Issue (14): 1721-1724. 上一篇    

研究简报

顺铂对卵巢癌COC1细胞总蛋白表达的影响研究

关松磊1,3，吕磊1，胡秀丽2，刘忠英2，宋凤瑞1，刘志强*,1   

  1. (1中国科学院长春应用化学研究所 长春质谱中心 长春 130022)
    (2吉林大学药学院 长春 130021)
    (3吉林农业大学生命科学学院 长春 130118)
  • 投稿日期:2010-09-28 修回日期:2011-03-15 发布日期:2011-03-24
  • 通讯作者: 刘志强 E-mail:liuzq@ciac.jl.cn
  • 基金资助:

    国家自然科学基金项目

Differential Expression of Total Proteins of Human Ovarian Cancer COC1 Cell Line Treated by cis-Diamminedichloroplatinum (DDP)

Guan Songlei1,3 Lü Lei1 Hu Xiuli2 Liu Zhongying2 Song Fengrui1 Liu Zhiqiang*,1   

  1. (1 Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022)
    (2 School of Pharmacy, Jilin University, Changchun 130021)
    (3 Department of Life Science, Jilin Agricultural University, Changchun 130118)
  • Received:2010-09-28 Revised:2011-03-15 Published:2011-03-24

PDF

1038

被引次数

3

对卵巢癌COC1细胞进行体外培养, 当培养至109/mL浓度时裂解细胞, 获得细胞总蛋白, 利用二维电泳对顺铂诱导前后的细胞内总蛋白质进行分离, 利用PDQuest 7.1.1专业分析软件对二维凝胶图谱进行差异比较, 利用基质辅助激光解析飞行时间质谱(MALDI-TOF-MS)进行质量分析, 最后进行生物信息学检索, 以寻找卵巢癌细胞发生、发展和凋亡的分子标记物. 经二维电泳图谱和质谱分析, 鉴定了11种蛋白, 4种蛋白表达量有差异, 7种蛋白在诱导前后表现为有和无的关系. 有5种是“分子伴侣”蛋白, 有4种蛋白与人体能量代谢有关. 蛋白功能分析提示卵巢癌细胞的蛋白质组表达存在差异, 这些差异蛋白对于寻找卵巢癌生物标志物, 开发新药提供很好的理论依据.

关键词: 卵巢癌, 顺铂, 二维电泳, 质谱分析, 分子标记物

Researcher cultured cells in vitro, when the concentration of cells is 109/mL, gained the total proteins within cells, separated the total-proteins by using two-dimensions electrophorese SDS-PAGE (SDS polyacrylamide gel electrophoresis), and analyzed the electrophoretogram with PDQuest 7.1.1 software, then detcted the mass by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF-MS) after in-gel digestion protocol, finally, identified the expressing proteins betweem ovarian cancer parent cell COC1 and subline treated by cisplatin, to search for the molecular makers of ovarian cancer cell in the phases of cytogenesis, growth and apoptosis. Resultly, 11 proteins had matches returned from the database search leading to the identification of these proteins, only about 7 proteins [all for the ovarian cancer cell COC1 treated by DDP (cis-diamminedichloroplatinum)] have a significant difference among these proteins. Meanwhile, 4 proteins are different expression on level, 5 proteins are molecular chaperones, 4 proteins are relative to energy metabolism. Function analysis demonstrate the expression diffence, these different proteins profit to study the bio-marker of ovairian cancer.

Key words: ovarian cancer, DDP, two-dimensions electrophoresis, mass spectrometry analysis, biomarker