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化学学报
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化学学报 ›› 2011, Vol. 69 ›› Issue (17): 2039-2046. 上一篇    下一篇

研究论文

基于CdTe量子点测定烟酸诺氟沙星的新方法研究

魏宏，王云云，宋尔群   

  1. (发光与实时分析教育部重点实验室 西南大学药学院 重庆 400715)
  • 投稿日期:2010-12-14 修回日期:2011-04-25 发布日期:2011-05-19
  • 通讯作者: 宋尔群 E-mail:eqsong@swu.edu.cn
  • 基金资助:

    国家自然科学基金;中央高校基本科研业务费;重庆市自然科学基金资助项目;西南大学博士基金

New Method for Detection of Norfloxacin Nicotinate Based on the CdTe Quantum Dots

WEI Hong, WANG Yun-Yun, SONG Er-Qun   

  1. (Key Laboratory of Luminescence and Real-Time Analysis of the Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing 400715)
  • Received:2010-12-14 Revised:2011-04-25 Published:2011-05-19

PDF

1872

被引次数

32 | 7

以巯基乙酸修饰的CdTe量子点(QDs)为荧光探针, 考察了不同条件下烟酸诺氟沙星对CdTe QDs荧光的猝灭作用. 发现在磷酸缓冲液中(PBS, pH 7.40, 0.10 mol/L), 其猝灭作用最强, 随后对其猝灭机理进行了讨论. 在此基础上建立了基于荧光猝灭法检测烟酸诺氟沙星含量的新方法, 该方法的线性范围为0.10～90 μg/mL, 线性方程为DF＝26.6206c－20.8273 (μg/mL), 相关系数r为0.997, 检测限(3S/N)为2.50×10－2 μg/mL. 应用此法检测兽药雏乐宝、烟酸氟哌酸和强效氟哌酸中烟酸诺氟沙星的含量, 百分标示量在93.0%～104.0%范围内变化, 相对标准偏差(RSD)≤4.2%|与常用的紫外分光光度法相比, 该法具有准确度高、灵敏度高且检测范围宽等优势, 显示出潜在的应用前景.

关键词: 量子点, 烟酸诺氟沙星, 荧光猝灭

The fluorescence quenching of norfloxacin nicotinate to thioglycolic acid stabilized CdTe quantum dots (QDs) has been systematically investigated by using CdTe QDs as a fluorescence probe. On the basis of that, a sensitive method for the determination of norfloxacin nicotinate was developed. The linear equation was DF＝26.6206c－20.8273 (μg/mL) and its linear range was 0.10～90 μg/mL with correlation coefficient of 0.997. The limit of detection was 2.50×10－2 μg/mL. Then the proposed new method was applied to measure the quantity of real sample (such as norfloxacin nicotinate solid powder), compared with the labeled amount, the accuracy was within 93.0%～104.0% and the RSD value for every sample five times determinations was or lower than 4.2%. This new method shows a bright application prospect for detection of norfloxacin nicotinate due to its several advantages such as accurate, high sensitivity, and wide detection range while compared with traditional ultraviolet spectrophotometry.

Key words: quantum dot, norfloxacin nicotinate, fluorescence quenching

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