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化学学报
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化学学报 ›› 2011, Vol. 69 ›› Issue (18): 2153-2158. 上一篇    下一篇

研究论文

双链DNA裂解-纳米金共振散射光谱探针检测痕量 UO22+  

张轶，梁爱惠*，周莲平，覃惠敏，欧阳辉祥，王鹏飞，蒋治良*   

  1. (广西师范大学环境与资源学院 广西环境工程与保护评价重点实验室 桂林 541004)
  • 投稿日期:2010-11-04 修回日期:2011-04-10 发布日期:2011-05-03
  • 通讯作者: 蒋治良 E-mail:zljiang@mailbox.gxnu.edu.cn
  • 基金资助:

    国家自然科学基金

Double Stranded DNA Cleaved-nanogold Resonance Scattering Spectral Probe for Assay of Trace UO2 2+

ZHANG Yi, LIANG Ai-Hui, ZHOU Lian-Ping, QIN Hui-Min, 欧Yang-Hui-Xiang , WANG Peng-Fei, JIANG Zhi-Liang   

  1. (Guangxi Key Laboratory of Environmental Engineering and Protection Assessment, School of Environment and Resource Sciences, Guangxi Normal University, Guilin 541004)
  • Received:2010-11-04 Revised:2011-04-10 Published:2011-05-03

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在pH 5.5的2-(N-吗啉)-乙磺酸缓冲液中, 底物DNA和酶DNA杂交形成双链DNA(dsDNA). 当加入 后, dsDNA中的底物DNA链被裂解, 释放的裂解链DNA吸附在金纳米粒子(GN)表面, 未吸附裂解链DNA的GN在NaCl存在下发生聚集, 在610 nm处有一最强的共振散射峰. 随着 的浓度增大, 体系中释放的裂解链DNA越多, 被裂解链DNA保护的GN越多, 聚集的GN越少, 导致610 nm处的共振散射峰下降, 溶液颜色由蓝色变红色. 浓度在0.67～60.3 nmol/L范围内与其共振散射峰降低值ΔI610 nm成线性, 回归方程为ΔI610 nm＝11.9C＋6.1, 相关系数为0.9972, 检出限为0.09 nmol/L . 该法用于废水中 的检测, 回收率在91.5%～95.7%之间.

关键词: 适配体, 双链DNA, 金纳米粒子, 共振散射光谱法

In pH 5.5 2-(N-morpholine)-ethyl sulfonic acid buffer solution and in the presence of NaCl, the substrate single stranded DNA hybridized with the DNA enzyme to form double stranded DNA (dsDNA) at 80 ℃. can cleaved the substrate strand of the dsDNA to produce short single stranded DNA that adsorbed on the surface of the gold nanoparticle to prevent its aggregation, and the uncombined gold nanoparticles aggregate to big particles that exhibited a resonance scattering (RS) peak at 610 nm. When the concentration increased, the short single stranded DNA increased, the combined gold nanoparticles increased, and the aggregated gold nanoparticles decreased, the RS intensity at 610 nm decreased. Under the selected conditions, the decreased RS intensity (ΔI610 nm) is linear to concentration in the range of 0.67～60.3 nmol/L, with a regression equation of ΔI610 nm＝11.9C＋6.1, a correlation coefficient of 0.9972, and a detection limit of 0.09 nmol/L . This method has been applied to determination of in wastewater, with satisfactory results.

Key words: aptamer, double stranded DNA, nanogold, resonance scattering spectral assay