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化学学报
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化学学报 ›› 2011, Vol. 69 ›› Issue (24): 3002-3006.DOI: 10.6023/A1101251 上一篇    下一篇

研究论文

一种基于PVC膜电极的电位式葡萄糖传感器的研制

康平利, 马小燕, 林觅, 郭淑杰, 刘新菲, 王建国*   

  1. (辽宁大学化学院 沈阳 110036)
  • 投稿日期:2011-01-25 修回日期:2011-07-05 发布日期:2011-08-15
  • 通讯作者: 王建国 E-mail:jgwang@lnu.edu.cn
  • 基金资助:

    辽宁省教育厅创新团队基金

Development of a Potentiometric Glucose Biosensor Based on a PVC Membrane Electrode

KANG Ping-Li, MA Xiao-Yan, LIN Mi, GUO Shu-Jie, LIU Xin-Fei, WANG Jian-Guo   

  1. (College of Chemistry, Liaoning University, Shenyang 110036)
  • Received:2011-01-25 Revised:2011-07-05 Published:2011-08-15
  • Contact: WANG Jian-Guo E-mail:jgwang@lnu.edu.cn

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被引次数

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以三庚基十二烷基碘代季铵盐为电活性物质的PVC膜电极, 对过氧化氢和六价钼的络合物具有良好的电位响应. 将葡萄糖氧化酶固定到PVC膜电极表面, 制备成电位式葡萄糖传感器. 在优化的实验条件下, 该传感器在葡萄糖浓度为2×10－4～5×10－3 mol/L范围内有线性响应, 检测下限为5×10－5 mol/L. 另外该传感器具有较高的稳定性和良好的选择性, 抗坏血酸、尿酸和一些氨基酸未对测定产生干扰.

关键词: PVC膜, 葡萄糖传感器, 六价钼, 电位式

Novel polyvinyl chloride (PVC) membrane electrodes containing triheptyl dodecyl ammonium iodide as electroactive materials exhibit excellent potentiometric response to the complex of hydrogen peroxide and hexavalent molybdenum. A potentiometric glucose biosensor was developed based on the PVC film electrode which was employed as a platform to immobilize Glucose oxidase (GOD). Under the optimization experiment conditions, experimental results show that the biosensor responds linearly to glucose in the range from 2×10－4 to 5×10－3 mol/L, the detection limit was 5×10－5 mol/L. In addition, the biosensor exhibits high stability and good selectivity against common interfering species such as ascorbic acid, uric acid and some kinds of amino acids.

Key words: PVC membrane, glucose sensor, hexavalent molybdenum, potentiometric