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化学学报
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化学学报 ›› 2011, Vol. 69 ›› Issue (24): 2981-2986.DOI: 10.6023/A1104171 上一篇    下一篇

研究论文

反射模式与线性模式MALDI-TOF MS联合分析荔枝果核缩合单宁

周海超*, 林益明*, 柴纬明, 魏淑东, 廖蒙蒙   

  1. (滨海湿地生态系统教育部重点实验室 厦门大学生命科学学院 厦门 361005)
  • 投稿日期:2011-04-17 修回日期:2011-07-31 发布日期:2011-10-11
  • 通讯作者: 林益明 E-mail:linym1967@yahoo.com.cn
  • 基金资助:

    教育部新世纪优秀人才支持计划;国家自然科学基金

Characterization of Condensed Tannins from Litchi Seed by Reflec-tron Modes and Linear Modes of MALDI-TOF MS

ZHOU Hai-Chao, LIN Yi-Ming, CHAI Wei-Ming, WEI Shu-Dong, LIAO Meng-Meng   

  1. (Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen 361005)
  • Received:2011-04-17 Revised:2011-07-31 Published:2011-10-11

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被引次数

13

利用不同模式的基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)分析荔枝果核缩合单宁. 在反射模式(Reflectron modes)下, 加速电压20.0 kV, 反射电压23.0 kV, 延时取出电压16.32 kV, 聚焦电压9.45 kV, 获得缩合单宁的准确结构单元组成和连接方式信息, 其为三聚体至十八聚体组成的A型原花青定多聚物. 在线性模式(Linear modes)下, 加速电压20.0 kV, 延时取出电压16.25 kV, 聚焦电压10.0 kV, 检测到十九聚体至二十七聚体的缩合单宁信号, 其更全面获取缩合单宁聚合度的分布信息. 综合两种模式, 荔枝果核缩合单宁是由三聚体至二十七聚体分布的A型原花青定多聚物. 探讨了一种联合反射模式与线性模式对植物单宁结构准确分析的基质辅助激光解吸电离飞行时间质谱方法.

关键词: 基质辅助激光解吸附飞行时间质谱, 反射模式, 线性模式, 荔枝果核, 缩合单宁

Condensed tannins from litchi seed were analyzed using reflectron modes and linear modes of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The reflectron modes (accelerating voltage, 20.0 kV|reflectron voltage, 23.0 kV|delayed extraction voltage, 16.32 kV|lens voltage, 9.45 kV) spectra obtained clear information on basic unit, interflavan linkage and polymerization degree extended from 3-mers to 18-mers. The linear modes (accelerating voltage, 20.0 kV|delayed extraction voltage, 16.25 kV|lens voltage, 10.0 kV) spectra further expanded the detectable polymers up to 27-mers. Litchi seed condensed tannins were A-type procyanidins polymer with as high as 27-mers. In the present study, reflectron modes coupled with linear modes was discussed as a potential method of MALDI-TOF MS in characterization of condensed tannins.

Key words: matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, reflectron mode, linear mode, litchi seed, condensed tannins