蠕虫状孔道介孔SiO2的合成及其固定化漆酶的活性

doi:10.6023/A12100836

化学学报 ›› 2013, Vol. 71 ›› Issue (04): 602-612.DOI: 10.6023/A12100836 上一篇下一篇

研究论文

蠕虫状孔道介孔SiO₂的合成及其固定化漆酶的活性

张剑波^a, 杨宇翔^b, 田美娟^b, 邓常健^b, 曹磊^b, 袁宏明^c

a 北京大学环境科学与工程学院北京 100871;
b 华东理工大学化学与分子工程学院上海 200237;
c 无机合成与制备化学国家重点实验室吉林大学化学学院长春 130012

投稿日期:2012-11-08 发布日期:2013-02-01
通讯作者: 杨宇翔 E-mail:yxyang@ecust.edu.cn

Synthesis of Worm-like Mesoporous Silica and Enzymatic Activity of the Immobilized Laccase

Zhang Jianbo^a, Yang Yuxiang^b, Tian Meijuan^b, Deng Changjian^b, Cao Lei^b, Yuan Hongming^c

a Department of Environmental Sciences, Peking University, Beijing 100871, China;
b School of Chemistry and Molecular Engineering, East China University of Science & Technology,Shanghai 200237, China;
c State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of Chemistry,Jilin University, Changchun 130012, China

Received:2012-11-08 Published:2013-02-01

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摘要/Abstract

以聚环氧乙烷-聚环氧丙烷-聚环氧乙烷三嵌段共聚物(P123)与离子型助表面活性剂{十六烷基三甲基溴化铵(CTAB)、十二烷基硫酸钠(SDS)或N-肉豆蔻酰-D-丙氨酸(C₁₄-D-A)}为结构导向剂, 合成了蠕虫状孔道的介孔SiO₂, 并在P123/C₁₄-D-A/TMAPS(N-三甲氧基硅丙基-N,N,N-三甲基氯化铵)合成反应体系中, 研究了扩孔剂聚乙二醇(PEG)-400的不同添加量对蠕虫状孔道的扩孔效果. 合成的产物分别用X-射线衍射(XRD)、扫描电镜(SEM)、透射电镜(TEM)和N₂吸附进行表征. 此外, 利用溶剂萃取法对扩孔后的蠕虫状介孔SiO₂进行处理, 得到不同孔径的季铵盐官能团化介孔SiO₂, 分别用于漆酶的固定化; 并对固定化酶的稳定性、所需的最佳pH值以及降解2,4-DCP的重复利用性进行了研究. 通过比较研究发现, 介孔SiO₂孔径与漆酶分子直径的匹配性是影响固定化漆酶比活及降解2,4-DCP效率的重要因素.

关键词: 蠕虫状介孔, 扩孔, 2,4-DCP, 降解效率, 重复利用性

Mesoporous silica with worm-like pore channels was synthesized using mixed P123 (polyethyleneoxide-polypropylene oxide-polyethylene oxide triblock copolymer EO₂₀PO₇₀EO₂₀) and ionic cosurfactant {CTAB, SDS or N-myristoyl-D-alanine (C₁₄-D-A)} as a structure-directing agent, and the pore-expanding effect of different amount of PEG-400 on the worm-like pore channels was studied. The synthesized products were characterized using X-ray diffraction, scanning electron microscopy, transmission electron microscopy, and nitrogen sorption analysis. Moreover, the worm-like pore-expanded mesoporous silica was extracted with solvent, and the obtained quaternary ammonium functionalized mesoporous silica with different pore size was applied to immobilize laccase respectively. The stability of immobilized laccase, the optimum pH value acquired and reusability for the degradation of 2,4-DCP were also studied. Through comparative studies, it is found that, matching of sizes between the enzyme molecule and the pore diameter of the mesoporous silica, is essential for specific activity of immobilized laccase and degradation efficiency of 2,4-DCP by immobilized laccase.

Key words: worm-like mesoporous silica, pore-expanding, 2,4-DCP, degradation efficiency, reusability

引用此文

张剑波, 杨宇翔, 田美娟, 邓常健, 曹磊, 袁宏明. 蠕虫状孔道介孔SiO₂的合成及其固定化漆酶的活性[J]. 化学学报, 2013, 71(04): 602-612.

Zhang Jianbo, Yang Yuxiang, Tian Meijuan, Deng Changjian, Cao Lei, Yuan Hongming. Synthesis of Worm-like Mesoporous Silica and Enzymatic Activity of the Immobilized Laccase[J]. Acta Chimica Sinica, 2013, 71(04): 602-612.

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蠕虫状孔道介孔SiO₂的合成及其固定化漆酶的活性

Synthesis of Worm-like Mesoporous Silica and Enzymatic Activity of the Immobilized Laccase

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