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化学学报
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化学学报 ›› 2004, Vol. 62 ›› Issue (12): 1162-1165. 上一篇    下一篇

研究论文

一种测定MDR肿瘤细胞内外阿霉素浓度的方法

张洪妍1, 沈朋2, 栾连军1, 程翼宇1   

  1. 1. 浙江大学药学院药物信息学研究所, 杭州, 310027;
    2. 浙江大学医学院附属第一医院, 杭州, 310027
  • 投稿日期:2003-12-02 修回日期:2004-02-26 发布日期:2014-02-17
  • 通讯作者: 程翼宇,E-mail:chengyy@zju.edu.cn E-mail:chengyy@zju.edu.cn
  • 基金资助:
    浙江省中医药科研计划(No.2003C076)资助项目.

An Approach to Determination of the Intracellular and Extracellular Concentrations of Doxorubicin for MDR Cancer Cells

ZHANG Hong-Yan1, SHEN Peng2, LUAN Lian-Jun1, CHENG Yi-Yu1   

  1. 1. Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310027;
    2. The First Hospital Attached to College of Medicine, Zhejiang University, Hangzhou 310027
  • Received:2003-12-02 Revised:2004-02-26 Published:2014-02-17

PDF

931

被引次数

49

研究提出K562/A细胞内外阿霉素浓度的反相高效液相色谱-荧光测定法.细胞内阿霉素浓度测定采用Lichrospher C18色谱柱,甲醇-0.01%醋酸(50:50,V/V)流动相,流速1.0 mL/min,柱温35℃;细胞外阿霉素浓度测定采用Lichrospher C18色谱柱,甲醇-0.01% 醋酸(55:45,V/V)流动相,流速0.8 mL/min,柱温35℃.荧光检测器波长λex=495 nm,λem=560 nm,均以盐酸柔红霉素为内标.研究结果表明,该方法简单、准确、线性范围宽、检测限低,精确度和回收率良好,可用于多药耐药肿瘤细胞内外阿霉素浓度的动态变化规律研究.

关键词: 液相色谱分析, 阿霉素, 多药耐药肿瘤细胞, K562/A细胞

A method using RP-HPLC with fluorescence detector was developed for determining the intracellular and extracellular concentrations of Doxorubicin (Dox) in K562/A cells. To analyze the intracellular concentration of Dox, a Lichrospher C18 column maintained at 35℃ was used, and the mobile phase consisted of methanol-0.01% acetic acid solution (50:50, V/V) at the flow rate of 1.0 mL/min. A Lichrospher C18 column maintained at 35℃ was used to analyze the extracellular concentration of Dox, and the mobile phase consisted of Methanol-0.01% acetic acid solution (55:45, V/V) at the flow rate of 0.8 mL/min. Daunorubicin was used as the internal standard, and the excitation wavelength and emission wavelength were 495 nm and 560 nm respectively. The proposed method has been verified to be simple, accurate and sensitive. It has low limit of determination, wide linear range, satisfied precision, and good mean recovery. For multi-drug resistance cancer cells, the dynamic status of the intracellular and extracellular concentrations of Dox could be investigated by using this method.

Key words: HPLC, Doxorubicin, MDR cancer cell, K562/A cell