在PBS缓冲体系中, 金纳米粒子与三聚氰胺形成粒径较大的聚集体, 导致共振瑞利散射(RRS)强度显著增强, 在一定条件下, 散射强度(ΔIRRS)与三聚氰胺浓度成正比, 由此建立了以金纳米粒子作光谱探针检测鲜牛奶中三聚氰胺的新方法. 在优化的实验条件下, 当三聚氰胺的浓度为3.3×10^－8～4.7×10^－7 mol/L时, 其线性关系为: ΔI _{RRS＝106.98C－28.279, R²＝0.9971, 检出限为2.7×10^－8 mol/L. 本方法金纳米粒子无需修饰, 在体系中仅加入一定量的单链寡聚核苷酸(T10), 实验表明该方法具有简便、快速、灵敏度高及选择性好等特点.}

A new resonance Rayleigh scattering method was developed for the determination of melamine in milk using gold nanoparticles as spectral probe. Under certain conditions, melamine can form bigger aggregate with gold nanoparticles, which resulted in great enhancement of resonance Rayleigh scattering. Based on optimizing various parameters, the linear dynamic range, the Regression equation, the linear correlation and the detection limit were 3.3×10^－8～4.7×10^－7 mol/L, ΔI_RRS＝106.98C－28.279, R²＝0.9971 and 2.7×10^－8 mol/L, respectively. In this method, the gold nanoparticles do not need to be decorated. Only an amount of single-strand oligonucleotide (T₁₀) need to be added to the system. The experiments showed that this developed method is practical, simple, sensitive and selective.