通过偶联剂γ-氨丙基三甲氧基硅烷(AMPS)的媒介, 通过表面引发接枝, 将功能单体甲基丙烯酸(MAA)接枝聚合在微米级硅胶微粒表面, 制得功能接枝微粒PMAA/SiO₂|采用本课题组建立的新型分子表面印迹技术, 以四咪唑(TM)为模型手性药物, 用其左旋对映体L-TM为模板分子, 乙二醇二缩水甘油醚(EGDE)为交联剂, 对接枝在硅胶表面的PMAA大分子链进行了分子印迹, 制备了对映体L-TM分子表面印迹材料MIP-PMAA/SiO₂. 探索了该印迹材料对外消旋体D,L-TM中两种对映体的拆分性能, 采用静态与动态两种方法考察了MIP-PMAA/SiO₂对模板分子L-TM的分子识别特性与结合性能. 实验结果表明, 印迹材料MIP-PMAA/SiO₂对L-TM分子具有特异的分子识别选择性与优良的结合亲和性, 相对于右旋对映体D-TM, 识别选择性系数为4.03, MIP-PMAA/SiO₂对外消旋体D,L-TM中的两种对映体表现出优良的拆分性能. 此外, 印迹材料MIP-PMAA/SiO₂也具有良好的解吸性能, 以稀NaOH水溶液为洗脱液, 20个床体积内解吸率即达到99.08%.

Via the coupling effect of γ-aminopropyltrimethoxysilane, methacrylic acid was graft-polymerized onto the surface of silica gel particles, obtaining the grafted particles PMAA/SiO₂. Then, the molecular imprinting towards the grafted PMAA was performed with one enantiomer of tetramisole (TM), L-TM, as template molecule and ethylene glycol diglycidyl ether (EGDE) as crosslinking agent by using the novel surface-molecular imprinting technique established by our research group, and the L-TM molecule-imprinted material MIP-PMAA/SiO₂ was prepared. With another enantiomer, D-TM, as a contrast compound, both static and dynamic methods were adopted to study the chiral recognition character and binding property of MIP-PMAA/SiO₂ for L-TM and to examine its ability to separate the two enantiomers in racemate. The experiment results show that MIP-PMAA/SiO₂ have specific recognition selectivity and excellent binding affinity for L-TM, and its selectivity coefficient for L-TM with respect to D-TM reaches 4.03, displaying fine ability to separate the two enantiomers. Besides, MIP-PMAA/SiO₂ has excellent elution property, and with a diluted aqueous solution of NaOH as the eluent, the desorption ratio of L-TM reaches 99.08% in 20 BV.