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2012 , Vol. 70 >Issue 03: 277 - 283

DOI: https://doi.org/10.6023/A1103022

研究论文

纳米Eu2O3 和Eu3+抑制人肝癌细胞HepG2 增殖的作用

  • 刘洁 ,
  • 魏春英 ,
  • 杨频
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  • 山西大学化学生物学与分子工程教育部重点实验室 分子科学研究所 太原 030006

收稿日期: 2011-03-02

  修回日期: 2011-09-27

  网络出版日期: 2011-11-01

基金资助

国家自然科学基金(Nos. 21041008, 21171108)、山西省自然科学基金(No. 2009011012-2)和山西省回国留学人员基金(2011-016)资助项目.

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Inhibitory Effect of Both Nano Eu2O3 and Eu3+ on Proliferation of HepG2 Cells

  • LIU Jie ,
  • WEI Chun-Ying ,
  • YANG Pin
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  • Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Molecular Science, Shanxi University, Taiyuan 030006

Received date: 2011-03-02

  Revised date: 2011-09-27

  Online published: 2011-11-01

Supported by

Project supported by the National Natural Science Foundation of China (Nos. 21041008, 21171108), Shanxi Provincial Fund for Natural Sciences (No. 2009011012-2) and the Research Project Supported by Shanxi Scholarship Council of China (No. 2011-016).

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摘要

稀土纳米氧化物和稀土化合物的生物效应已引起人们的广泛关注. 利用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐(MTT), 流式细胞术法和激光共聚焦显微镜初步探讨了纳米Eu2O3 和Eu3+对体外培养的人肝癌细胞HepG2 生长的影响.结果发现, 较低浓度的纳米Eu2O3 对细胞生长没有明显影响, 浓度达到600 μg·mL-1 作用癌细胞仅24 h, 细胞就停止分裂,表现为细胞被阻滞在S 期, 大量细胞坏死. 利用激光共聚焦显微镜观察到纳米Eu2O3 能进入活的HepG2 细胞中. 而Eu3+则在较低含量, 即≤100 μmol·L-1 时能较弱地抑制癌细胞的生长, 细胞被阻滞在G0/G1 期, 并诱导细胞发生凋亡.

关键词： 纳米Eu2O3; Eu3+; HepG2 细胞; 凋亡; 稀土

本文引用格式

刘洁 , 魏春英 , 杨频 . 纳米Eu2O3 和Eu3+抑制人肝癌细胞HepG2 增殖的作用[J]. 化学学报, 2012 , 70(03) : 277 -283 . DOI: 10.6023/A1103022

Abstract

The biological effects of rare earth elements and rare earth nanoparticles have aroused an extensive interest during the past few years. In vitro culture conditions, we reported the impact of nano Eu2O3 and Eu3+ on the HepG2 cells using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), flow cytometry and laser scanning confocal microscopy. The results suggested that the nano Eu2O3 at low concentrations does not make an obvious impact on cell growth whereas it can suspend cell division, and the percentage of cells in G2/M phase increase gradually and lead to necrosis at higher concentrations (≥600 μg·mL-1). In addition, nano Eu2O3 can be internalized by live HepG2 cells. Eu3+ at low concentrations (≤100 μmol·L-1) can slightly inhibit the growth of HepG2 cells by arresting the ratio of G0/G1 phase cells and triggerring apoptosis.

Key words： nano Eu2O3; Eu3+; HepG2 cells; apoptosis; rare earth

参考文献

1 Chen, L.-J. Sci. Technol. Inf. 2006, 32, 29 (in Chinese). (陈丽娟, 科技资讯, 2006, 32, 29.)

2 Fu, L.-J.; Li, J.-X.; Yang, X.-G.; Wang, K. J. Biol. Inorg.Chem. 2009, 14, 219.  

3 Shigematsu, T. Ther. Apheresis Dial. 2008, 12, 55.  

4 Jiang, W.-H.; Chen, D.; Meng, X.-T. J. Chin. Rare Earth Soc. 2004, 22, 292 (in Chinese). (姜文华, 陈东, 孟晓婷, 中国稀土学报, 2004, 22, 292.)

5 Sahoo, S. K.; Labhasetwar, V. Drug Disc. Today 2003, 8, 1112.  

6 Zhu, R.-R.; Wang, S.-L.; Chen, X.-P.; Sun, X.-H.; Zhang, R.; Yao, S.-D. Acta Chim. Sinica 2006, 64, 2161 (in Chinese). (朱融融, 汪世龙, 陈小平, 孙晓宇, 张蕤, 姚思德, 化学 学报, 2006, 64, 2161.)

7 Guan, H.-F.; Liao, L.-W. Guizhou Chem. Ind. 2004, 29, 30 (in Chinese). (管海凤, 廖列文, 贵州化工, 2004, 29, 30.)

8 Chen, Y.; Yang, L.-S.; Feng, C.; Wen, L.-P. Biochem. Biophys. Res. Commun. 2005, 337, 52.  

9 Park, E. J.; Choi, J.; Park, Y. K.; Park, K. Toxicology 2008, 245, 90.  

10 Chen, G. Rare Earth Inf. 2002, 12, 12 (in Chinese). (陈刚, 稀土信息, 2002, 12, 12.)

11 Liu, J.; Wei, C.-Y.; Yang, P. J. Chin. Rare Earth Soc. 2011, 29, 112 (in Chinese). (刘洁, 魏春英, 杨频, 中国稀土学报, 2011, 29, 112.)

12 Zhou, X.-P.; Hou, H.-Z.; Li, Y.-Q.; Yang, P. Acta Chim. Sinica 2011, 69, 106 (in Chinese). (周雪萍, 侯惠智, 李英奇, 杨频, 化学学报, 2011, 69, 106.)

13 Li, Y. M.S. Thesis, Jilin University, Changchun, 2010 (in Chinese). (李阳, 硕士论文, 吉林大学, 长春, 2010.)  

14 Yu, L. M.S. Thesis, University of Science and Technology of China, Hefei, 2009 (in Chinese). (于乐,硕士论文, 中国科学技术大学, 合肥, 2009.)  

15 Jin, H.-F.; Yuan, L.; Qiu, L.; Wu, K. Orthopaedic Biomechanics Materials and Clinical Study, 2009, 6, 33 (in Chinese). (金华芳, 袁琳, 邱乐, 武奎, 生物骨科材料与临床研究, 2009, 6, 33.)

16 Dai, Y.-C.; Li, J.; Yu, L.; Dai, G.; Hu, A.-G.; Yuan, L.-Y.; Wen, Z. In Vitro Cell. Dev. Biol. Anim. 2002, 38, 373.  

17 Shi, P.; Huang, Z.-W. Biometals 2005, 18, 89.  

18 Zhang, Y.; Fu, L.-J.; Li, J.-X.; Yang, X.-G.; Yang, X.-D.; Wang, K. Biometals 2009, 22, 511.  

19 Yang, B.-S.; Harris, W.-R. Acta Chim. Sinica 1999, 57, 503 (in Chinese). (杨斌盛, Harris, W. R. 化学学报, 1999, 57, 503.)
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