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2012 , Vol. 0 >Issue 05: 617 - 623

DOI: https://doi.org/10.6023/A1107302

研究论文

有序中孔炭/四氧化三铁复合材料的制备及其用于血红蛋白的电化学研究

  • 郑静 ,
  • 赵家昌 ,
  • 龚晨 ,
  • 刘萍 ,
  • 陈瑜 ,
  • 黄焕 ,
  • 何品刚 ,
  • 方禹之
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  • a 上海工程技术大学化学化工学院 上海 201620;
    b 华东师范大学化学系 上海 200062

收稿日期: 2011-07-30

  修回日期: 2012-01-12

  网络出版日期: 2012-03-17

基金资助

国家自然科学基金(No. 20675031)和上海市教委(No. 09YZ374)资助项目.

收起

Preparation of the Ordered Mesoporous Carbon/Fe3O4 and Its Application for the Direct Electrochemistry of Hemoglobin

  • Zheng Jing ,
  • Zhao Jiachang ,
  • Gong Chen ,
  • Liu Ping ,
  • Chen Yu ,
  • Huang Huan ,
  • He Pingang ,
  • Fang Yuzhi
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  • a College of Chemistry and Chemical Engineering, Shanghai University of Engineering Science, Shanghai 201620;
    b Department of Chemistry, East China Normal University, Shanghai 200062

Received date: 2011-07-30

  Revised date: 2012-01-12

  Online published: 2012-03-17

Supported by

Project supported by the National Natural Science Foundation of China (No. 20675031) and Innovation Program of Shanghai Municipal Education Commission (No. 09YZ374).

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摘要

采用微湿含浸法制备了有序中孔炭/四氧化三铁磁性材料. 采用透射电镜和X 射线衍射对复合材料进行了表征.将血红蛋白(Hb)固定于材料表面, 对其直接电化学行为进行了研究, 结果表明Hb 在该材料内仍保持了其生物活性, 在pH＝7.0 的PBS 缓冲液中, 血红蛋白表现出一对峰形良好的准可逆氧化还原峰, 为Hb 的Fe(III)/Fe(II)电对的特征峰, 求出式电位E0'为－0.306 V, 电子转移数为n＝1.226, 电荷传递系数为α＝0.51, 表观异相电子转移速率常数为KS＝0.0144s-1. 在3.00×10-6 到1.50×10-4 mol/L 浓度范围内, 血红蛋白的浓度与其响应电流呈良好的线性关系, 线性相关系数为R＝0.9924, 最低检测限为0.270×10-6 mol/L.

关键词： 有序中孔炭; 四氧化三铁; 制备; 血红蛋白; 直接电化学

本文引用格式

郑静 , 赵家昌 , 龚晨 , 刘萍 , 陈瑜 , 黄焕 , 何品刚 , 方禹之 . 有序中孔炭/四氧化三铁复合材料的制备及其用于血红蛋白的电化学研究[J]. 化学学报, 2012 , 0(05) : 617 -623 . DOI: 10.6023/A1107302

Abstract

The novel magnetic composites of ordered mesoporous carbon/Fe3O4 were prepared with a micro-wetness impregnation method. The composites were characterized by transmission electron microscope and X-ray diffraction. Hemoglobin was immobilized onto the surface of the composites, and was shown to keep its biological activity well. The electrochemical behavior of Hb was studied, and a pair of well-defined, quasi-reversible cyclic voltammetric redox peaks was obtained, which was characteristics of Hb Fe(III)/Fe(II) redox couples. The formal potential (E0'), the electron transfer number (n), and transfer coefficient (α) were calculated to be －0.306 V, 1.226 and 0.51, respectively. The average apparent heterogeneous electron transfer rate constant (KS) obtained for Hb was 0.0144 s-1. Linear sweep voltammetric measurement of Hb indicated that the peak currents of Hb were linear to its concentration ranging from 3.00×10-6 to 1.50×10-4 mol/L with the detection limits of 0.270×10-6 mol/L.

Key words： ordered mesoporous carbon; ferriferrous oxide; preparation; hemoglobin; direct electrochemistry

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