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2012 , Vol. 70 >Issue 08: 1008 - 1012

DOI: https://doi.org/10.6023/A1108021

研究论文

纳米SnSe2 空心球上乙酰胆碱酯酶的固定及辛硫磷传感器的构建

  • 张灿 ,
  • 张金星 ,
  • 王坤 ,
  • 戴志晖
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  • 江苏省生物功能材料重点实验室 南京师范大学化学与材料科学学院 南京 210097

收稿日期: 2011-08-02

  修回日期: 2011-11-25

  网络出版日期: 2011-12-14

基金资助

国家自然科学基金(No. 21175069)、教育部新世纪优秀人才支持计划(No. NCET-09-0159)和江苏省优势学科资助项目.

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Immobilization of Acetylcholinesterase on SnSe2 Hollow Spheres for Sensitive Detection of Phoxim

  • Zhang Can ,
  • Zhang Jinxing ,
  • Wang Kun ,
  • Dai Zhihui
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  • Jiangsu Key Laboratory of Biofunctional Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210097

Received date: 2011-08-02

  Revised date: 2011-11-25

  Online published: 2011-12-14

Supported by

Project supported by the National Natural Science Foundation of China (No. 21175069), the Program for New Century Excellent Talents in University (No. NCET-09-0159) and the Priority Academic Program Development of Jiangsu Higher Education Institutions.

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摘要

描述了一种将乙酰胆碱酯酶固定在SnSe2 空心球上检测辛硫磷的简单方法, 用水热法合成了SnSe2 空心球, 并用透射电镜对其表征. 固定的乙酰胆碱酯酶能保持其生物学活性, 催化乙酰胆碱为胆碱, 胆碱被氧化产生可检测的信号.基于辛硫磷对乙酰胆碱酯酶活性有抑制作用这个机理, 在理想条件下, 这种传感器对辛硫磷检测的线性范围是0.008～56 μg/mL, 检测限为0.004 μg/mL. 这种新型的传感器有很好的稳定性和重现性. 这项工作表明SnSe2 空心球可以作为固定乙酰胆碱酯酶的理想载体并用于构建相应的传感器.

关键词： 乙酰胆碱酯酶; SnSe2 空心球; 辛硫磷

本文引用格式

张灿 , 张金星 , 王坤 , 戴志晖 . 纳米SnSe2 空心球上乙酰胆碱酯酶的固定及辛硫磷传感器的构建[J]. 化学学报, 2012 , 70(08) : 1008 -1012 . DOI: 10.6023/A1108021

Abstract

A simple method for immobilization of acetylcholinesterase (AChE) on SnSe2 hollow spheres for detection of phoxim was described. SnSe2 hollow spheres were prepared by hydrothermal methods and were characterized by TEM. The immobilized AChE retained its biological activity well and could catalyze the hydrolysis of acetylthiocholine to form thiocholine, which was then oxidized to produce detectable signal. Based on the inhibition toward the enzymatic activity of AChE by phoxim, under optimal conditions, the sensors could be used for the determination of phoxim ranging from 0.008 to 56 μg/mL with the detection limit of 0.004 μg/mL. The developed phoxim biosensors exhibited good stability and reproducibility. This work demonstrated that SnSe2 hollow sphere could be served as an ideal carrier for immobilization of AChE to fabricate corresponding biosensor.

Key words： acetylcholinesterase; SnSe2 hollow spheres; phoxim

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