SIOC English
化学学报
高级检索

化学学报 >

2012 , Vol. 70 >Issue 11: 1283 - 1288

DOI: https://doi.org/10.6023/A1201124

研究论文

碳量子点对基于能量转移电化学发光DNA传感体系影响的研究

  • 杨帆 ,
  • 王伶俐 ,
  • 郭志慧
展开
  • 陕西省生命分析化学重点实验室 陕西师范大学化学化工学院 西安 710062

收稿日期: 2012-01-12

  修回日期: 2012-02-27

  网络出版日期: 2012-02-27

基金资助

国家自然科学基金(No. 20905044)资助项目.

收起

Study on the Influence of Carbon Quantum Dots on Electrogenerated Chemiluminescence Energy Transfer Sensing System of DNA

  • Yang Fan ,
  • Wang Lingli ,
  • Guo Zhihui
Expand
  • Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062

Received date: 2012-01-12

  Revised date: 2012-02-27

  Online published: 2012-02-27

Supported by

Project supported by the National Natural Science Foundation of China (No. 20905044).

Fold

摘要

实验发现在鲁米诺-结晶紫能量转移电化学发光体系中再加入碳量子点后, 体系的灵敏度和重现性均提高. 本实验中, 探究出用能量转移电化学发光分析法区分单双链DNA 的优化条件. 在优化条件下, 测定目标DNA 的线性范围为1.0×10-11～1.0×10-7 mol/L, 相关系数为0.9881, 检出限为4.0×10-12 mol/L, 并且讨论了DNA 与碳量子点可能的作用方式. 本法操作方便, 灵敏度高.

关键词： 电化学发光; 碳量子点; DNA 检测; 鲁米诺; 结晶紫

本文引用格式

杨帆 , 王伶俐 , 郭志慧 . 碳量子点对基于能量转移电化学发光DNA传感体系影响的研究[J]. 化学学报, 2012 , 70(11) : 1283 -1288 . DOI: 10.6023/A1201124

Abstract

The effect of carbon quantum dots on the electrogenerated chemiluminescence (ECL) energy transfer sensing system for the determination of DNA was investigated. The results showed that the signal- to-noise ratio of luminol-crystal violet ECL system was increased and its reproducibility was improved by the addition of carbon quantum dots. The conditions of the detection of DNA were optimized. Under optimal conditions, the linear range for the determination of target DNA was 1.0×10-11～1.0×10-7 mol/L, the correlation coefficient was 0.9881 and the detection limit was 4.0×10-12 mol/L. The possible interaction mechanism between DNA and carbon quantum dots was also discussed. This method was convenient and showed high sensitivity.

Key words： electrogenerated chemiluminescence; carbon quantum dots; DNA detection; luminol; crystal violet

参考文献

1 Huang, W.; Fernando, S.; Allard, L. F.; Sun, Y.-P. Nano Lett. 2003, 3(4), 565.

2 Hasheminezhad, M.; Fleischner, H.; McKay, B. D. Chem. Phys. Lett. 2008, 464(1-3), 118.

3 Ramachandran, C. N.; Sathyamurthy, N. J. Phys. Chem. A2007, 111(30), 6901.

4 Cook, S. M.; Aker, W. G.; Rasulev, B. F.; Hwang, H. M.; Leszczynski, J.; Jenkins, J. J.; Shockley, V. J. Hazard. Mater.2010, 176(1-3), 367.

5 Kamanina, N. V.; Serov, S. V.; Savinov, V. P. Tech. Phys. Lett. 2010, 36(1), 40.

6 Fu, C. C.; Lee, H. Y.; Chen, K.; Lim, T. S.; Wu, H.-Y.; Lin, P.-K.; Wei, P.-K.; Tsao, P. H.; Chang, H.-C.; Fann, W. Proc. Natl. Acad. Sci. U. S. A. 2007, 104(3), 727.  

7 Gunningham, G.; Panich, A. M.; Shames, A. I.; Petrov, I.; Shenderova, O. Diamond Relat. Mater. 2008, 17(4-5), 650.  

8 Kong, X.-L. Chin. Optics Lett. 2008, 6(6), 417.  

9 Novikov, N. V.; Bogatyreva, G. P. J. Superhard Mater.2008, 30(2), 73.

10 Sun, Y.-P.; Zhou, B.; Lin, Y. J. Am. Chem. Soc. 2006, 128,7756.  

11 Kang, J.-L.; Nash, P.; Li, J.-J.; Shi, C.-S.; Zhao, N.-Q. Nanotechnology 2009, 20(23), 235607.

12 Latorre, N.; Villacampa, J. I.; Ubieto, T.; Romeo, E.; Royo, C.; Borgna, A.; Monzon, A. Top. Catal. 2008, 51(l-4), 158.

13 Barcena, J.; Maudesa, J.; Coletoa, J.; Baldonedo J. L.; Gomez de Salazar, J. M. Compos. Sci. Technol. 2008, 68(6),1384.

14 Pico, F.; Ibaez, J.; Lillo-Rodenas, M. A.; Linares-Solano, A.; Rojas, R. M.; Amarilla, J. M.; Rojo, J. M. J. Power Sources 2008, 176(1), 417.  

15 Xu, X.-Y.; Ray, R.; Gu, Y. L.; Ploehn, H. J.; Geatheart, L.; Raker, K.; Scrivens, W. A. J. Am. Chem. Soc. 2004,126(40), 12736.  

16 Cao, L.; Wang, X.; Meziani, M. J.; Lu, F. S.; Wang, H. F.; Luo, P. G.; Lin, Y.; Harruff, B. A.; VecaL, M.; Murray, D.; Xie, S.-Y.; Sun, Y.-P. J. Am. Chem. Soc. 2007, 129, 11318.  

17 Bai, W.-J.; Zheng, H.-Z.; Long, Y.-J.; Mao, X.-J.; Gao, M.; Zhang, L.-Y. Anal. Sci. 2011, 27(3), 243.  

18 Kong, D.-M.; Ma, Y.-E.; Wu, J.; Shen, H.-X. Chem. Eur. J.2009, 15, 901.  

19 Kong, D.-M.; Guo, J.-H.; Yang, W.; Ma, Y.-E.; Shen, H.-X. Biosens. Bioelectron. 2009, 25, 88.  

20 Zhu, H.; Wang, X.-L.; Li, Y.-L.; Wang, Z.-J.; Yang, F.; Yang, X.-R. Chem. Commun. 2009, 5118.  

21 Li, J.-Q.; Wei, Y.-L.; Dong, C. J. Anal. Sci. 2010, 26, 35 (in Chinese). (李建晴, 卫艳丽, 董川, 分析科学学报, 2010, 26, 35.)

22 Yang, R.-H.; Jin, J.-Y.; Chen, Y.; Shao, N.; Kang, H.-Z.; Xiao, Z.-Y.; Tang, Z.-W.; Wu, Y.-R.; Zhu, Z.; Tan, W.-H. J. Am. Chem. Soc. 2008, 130, 8351.  

23 Zheng, M.; Jagota, A.; Semke, E. D.; Diner, B. A.; Mclean, R. S.; Lustig, S. R.; Richardson, R. E.; Tassi, N. G. Nat. Mater. 2003, 2, 338.  

24 Ouyang, X.-Y.; Yu, R.-Q.; Jin, J.-Y.; Li, J.-S.; Yang, R.-H.; Tan, W.-H.; Yuan, J.-L. Anal. Chem. 2011, 83, 782.  

25 Ouyang, X.-Y.; Liu, J.-H.; Li, J.-S.; Yang, R.-H. Chem. Commun. 2012, 48, 88.  

26 Gong, H.-P.; Liu, S.-P.; Yin, P.-F.; Yan, S.-G.; Fan, X.-Q.; He, Y.-Q. Acta Chim. Sinica 2011, 69, 2843 (in Chinese). (龚会平, 刘绍璞, 殷鹏飞, 闫曙光, 范小青, 何佑秋, 化 学学报, 2011, 69, 2843.)

Options
文章导航

/

〈 〉