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2012 , Vol. 70 >Issue 20: 2127 - 2132

DOI: https://doi.org/10.6023/A12080499

研究通讯

表面引发的DNA杂交链式反应的石英晶体微天平研究

  • 王萍 ,
  • 葛志磊 ,
  • 裴昊 ,
  • 王丽华 ,
  • 樊春海
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  • 中国科学院上海应用物理研究所物理生物学实验室 上海 201800

收稿日期: 2012-08-03

  网络出版日期: 2012-09-05

基金资助

项目受国家自然科学基金(No. 21075128)和上海市科委(No. 10QA1408200)资助.

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Quartz Crystal Microbalance Studies on Surface-Initiated DNA Hybridization Chain Reaction

  • Wang Ping ,
  • Ge Zhilei ,
  • Pei Hao ,
  • Wang Lihua ,
  • Fan Chunhai
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  • Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800

Received date: 2012-08-03

  Online published: 2012-09-05

Supported by

Project supported by the National Natural Science Foundation of China (No. 21075128) and Shanghai Municipal Commission for Science and Technology (No. 10QA1408200).

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摘要

通过石英晶体振荡技术研究了杂交链式反应这种核酸扩增的方法. 石英晶体微天平可以表征在晶体和溶液的界面上的DNA层, 并获得粘性穿透深度这一重要参数. 根据石英晶体表面吸附质量和振荡频率之间的关系, 我们测量了表面引发的杂交链式反应的动力学过程, 并获得界面上的粘度、剪切模量等参数. 这一工作为研究固液界面上核酸反应过程, 特别是杂交链式反应的机制提供了新的途径.

关键词： 杂交链式反应; 石英晶体微天平; DNA; 粘度; 界面; 穿透深度

本文引用格式

王萍 , 葛志磊 , 裴昊 , 王丽华 , 樊春海 . 表面引发的DNA杂交链式反应的石英晶体微天平研究[J]. 化学学报, 2012 , 70(20) : 2127 -2132 . DOI: 10.6023/A12080499

Abstract

The hybridization chain reaction (HCR), a type of nucleic acid amplification method, was studied by a quartz crystal resonator technique. We probed the properties of the boundary DNA layer with a thickness of the viscous penetration depth at the crystal-solution interface. According to the relationship between the change of resonant frequency and the mass adsorbed on the crystal surface, the kinetics of surface-initiated HCR was measured, as well as the viscosity and the shear modulus of H1/H2 solution with different concentrations near a solid substrate. This work provides a new method for studying nucleic acid reactions at solid surface and mechanistic insight for HCR-based amplification and biodetection.

Key words： hybridization chain reaction; quartz crystal microbalance; DNA; viscosity; interface; penetration depth

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