SIOC English
化学学报
高级检索

化学学报 >

2013 , Vol. 71 >Issue 9: 1293 - 1300

DOI: https://doi.org/10.6023/A13040401

研究论文

β-谷甾醇、豆甾醇与DPPC相互作用的单分子膜研究

  • 杨静 ,
  • 孙润广 ,
  • 郝长春 ,
  • 乔进京 ,
  • 何光晓
展开
  • 陕西师范大学物理学与信息技术学院生物物理与生物医学工程研究室 西安 710062

收稿日期: 2013-04-12

  网络出版日期: 2013-05-16

基金资助

项目受国家自然科学基金(No. 20772077)、教育部高等学校博士学科点专项科研基金(No. 200807180001)、陕西省自然科学基础研究计划(No. 2012JQ1002)和中央高校基本科研业务费专项(No. GK201102029)资助.

收起

A Monolayer Study on the Interfacial Behavior of DPPC with β-sitosterol and Stigmasterol

  • Yang Jing ,
  • Sun Runguang ,
  • Hao Changchun ,
  • Qiao Jinjing ,
  • He Guangxiao
Expand
  • Laboratory of Biophysics and Biomedical Engineering, College of Physics and Information Technology, Shaanxi Normal University, Xi'an 710062

Received date: 2013-04-12

  Online published: 2013-05-16

Supported by

Project supported by the National Natural Science Foundation of China (No. 20772077), the Doctoral Fund of Ministry of Education of China (No. 200807180001), the Natural Science Basic Research Plan in Shaanxi Province of China (No. 2012JQ1002) and the Fundamental Research Funds for the Central Universities (No. GK201102029).

Fold

摘要

植物甾醇对生物膜具有重要的调节作用, 甾醇尾部的饱和程度对生物膜性质的影响一直是个热点问题. 通过LB技术和原子力显微镜分别研究了尾部是单键的β-谷甾醇(β-sito)和尾部是双键的豆甾醇(stig)与二棕榈酰磷脂酰胆碱(DPPC)的相互作用, 深入分析了β-谷甾醇、豆甾醇对DPPC单分子膜液态扩张-液态聚集(LE-LC)相变过程的影响. 实验结果表明: β-谷甾醇、豆甾醇添加到DPPC单分子膜中, 膜的压缩性以及分子的排列都发生了较大变化. 当植物甾醇含量Xsterols=0.8时, β-谷甾醇、豆甾醇与DPPC相互作用具有相同的规律, 过量分子面积和过量吉布斯自由能均为负值, 说明分子间的吸引力比排斥力更强; 在低浓度下, Xsterols=0.2, 0.4时, 两种系统的过量分子面积和过量吉布斯自由能呈现出明显的区别, 尾部是单键的β-谷甾醇和DPPC混合单分子膜的排斥力更强烈, 而尾部是双键的豆甾醇和DPPC混合单分子膜的吸引力更强烈, 说明尾部是双键的豆甾醇比尾部是单键的β-谷甾醇更容易和DPPC发生凝聚, AFM数据进一步证实了这些结果.

关键词： 植物甾醇; 相互作用; 相变; 压缩性; AFM

本文引用格式

杨静 , 孙润广 , 郝长春 , 乔进京 , 何光晓 . β-谷甾醇、豆甾醇与DPPC相互作用的单分子膜研究[J]. 化学学报, 2013 , 71(9) : 1293 -1300 . DOI: 10.6023/A13040401

Abstract

It is known that sterols are key components of cellular membranes, which can modulate membrane properties and induce influence on the organization of membrane phospholipids. Howerer, the influence of these sterols on membrane properties sees to be unclear. It is worth studying. The interactions between β-sitosterol and stigmasterol and dipalmitoyl phosphatidycholine (DPPC) in mixed monolayers were studied by langmuir technique and atomic force microscopy (AFM). The influence of β-sitosterol and stigmasterol effects on properties of DPPC membrane on phase transition from the liquid expanded (LE) to liquid condensed (LC) state were investigated and the condensing and ordering potency of binary monolayers containing β-sitosterol and stigmasterol were thoroughly analyzed. Based on the analysis of mixing values as well as AFM images it was evidenced that the effect of the plant sterol depends on the composition of model membrane. The results show that the addition of the plant sterols into model membrane modifies the condensation, ordering and interactions in the system. Moreover, at their higher concentration Xsterols=0.8, the values of the excess molecular area and excess Gibbs enerqy displayed negative values, which indicate that between molecules in the mixed monolayer exist stronger attraction than in the respective one-component films and suggest β-sitosterol and stigmasterol in the mixed system have the similar effects on its properties. Examination of the excess free energy of mixing reveals an enhanced stability of binary monolayers. At lower content, Xsterols=0.2, 0.4, the values of the excess molecular area and excess Gibbs energy suggested stronger attractive interaction between stigmasterol and DPPC, while an intermolecular stronger repulsion interaction between β-sitosterol and DPPC. It shows that stigmasterol was found to modify the properties of model membrane more strongly than β-sitosterol. It reflects that side-chain can reduce packing effectiveness, increased tilt of the molecules with respect to the monolayer plane, and the presence of the double bond reduces the hydrophobicity of the branched hydrocarbon tail of stigmasterol, which could cause strols to have long axis parallel to the interface. The result of AFM supported the theoretic analysis.

Key words： phytosterol; interaction; phase transition; condensation; atomic force microscopy (AFM)

参考文献

[1] Zappel, N. F.; Panstruga, R. Curr. Opin. Plant Biol. 2008, 11, 632.
[2] Pike, L. J. Biochem. J. 2004, 378, 285.
[3] Roche, Y.; Gerbeau-Pissot, P.; Buhot, B.; Thomas, D.; Bonneau, L.; Gresti, J.; Mongrand, S.; Perrier-Cornet, J. M.; Simon-Plas, F. FASEB J. 2008, 22, 3980.
[4] Ostlund, R. E. Jr. Lipids 2007, 42, 41.
[5] Kritchevsky, D.; Chen, S. C. Nutr. Res. 2005, 25, 414.
[6] Piironen, V.; Lindsay, D. G.; Miettinen, T. A.; Toivo, J.; Lampi, A. M. J. Sci. Food Agric. 2000, 80, 939.
[7] Bernsdorff, C.; Winter, R. J. Phys. Chem. B 2003, 107, 10658.
[8] Hendriks, H. F. J.; Brink, E. J.; Meijer, G. W.; Princen, H. M. G.; Ntanios, F. Y. Eur. J. Clin. Nutr. 2003, 57, 681.
[9] Ketomaki, A. M.; Gylling, H.; Antikainen, M.; Siimes, M. A.; Miettinen, T. A. J. Pediatr. 2003, 142, 524.
[10] Katarzyna, H. W. Colloids Surf. B: Biointerfaces 2012, 91, 226.
[11] Pianese, P.; Salvia, G.; Campanozzi, A.; D’Apolito, O.; Dello Russo, A.; Pettoello-Mantovani, M.; Corso, G. J. Pediatr. Gastroenterol. Nutr. 2008, 47, 645.
[12] Katarzyna, H. W.; Patrycja, D. Biochim. Biophys. Acta 2012, 1818, 2184.
[13] Deng, S. P.; Ouyang, J. M. Acta Chim. Sinica 2007, 65, 683. (邓穗平, 欧阳健明, 化学学报, 2007, 65, 683.)
[14] Chang, Y. G.; Sun, R. G.; Hao, C. C.; Xue, H. J.; Han, T.; Wang, X. M. Acta Chim. Sinica 2010, 68, 2181. (常怡光, 孙润广, 郝长春, 薛慧君, 韩涛, 王小梅, 化学学报, 2010, 68, 2181.)
[15] Sun, R. G.; Zhang, J. Atom. Mol. Phys. 2003, 20, 85. (孙润广, 张静, 原子与分子物理学报, 2003, 20, 85.)
[16] Sun, R. G.; Zhang, J.; Wang, Y. C. Prog. Biochem. Biophys. 2000, 27, 296. (孙润广, 张静, 王永昌, 生物化学与生物物理进展, 2000, 27, 296.)
[17] Wang, F. Y.; Sun, R. G.; Chen, Y. Y.; Wang, X. M. Acta Chim. Sinica 2012, 70, 668. (王夫雨, 孙润广, 陈莹莹, 王小梅, 化学学报, 2012, 70, 668.)
[18] Komada, M.; Shibata, O.; Nakamura, S.; Lee, S.; Sugihara, G. Colloids Surf. B: Biointerfaces 2004, 33, 211.
[19] Katarzyna, H. W.; Patrycja, D. Biophys. Chem. 2006, 123, 154.
[20] Bordi, F.; Cametti, C.; Venanzio, C. D.; Sennato, S.; Zuzzi, S. Colloids Surf. B 2008, 61, 304.
[21] Lee, Y. L.; Lin, J. Y.; Chang, C. H. T. J. Colloid Interface Sci. 2006, 296, 647.
[22] Katarzyna, H. W.; Pawe?, W. Chem. Phys. Lipids 2007, 150, 66.
[23] Dynarowicz, A. P.; Kita, K. Adv. Colloid Interface Sci. 1999, 79, 1.
[24] Hac-Wydro, K.; Dynarowicz-?atka, P.; Grzybowska, J.; Borowski, E. Thin Solid Films 2008, 516, 1197.
[25] Su, Y. L.; Li, Q. Z.; Chen, L.; Yu, Z. W. Colloids Surf. A 2007, 293, 123.
[26] Hao, C. C.; Sun, R. G.; Zhang, J.; Chang, Y. G.; Niu, C. L. Sci. China, Ser. B 2008, 38, 1018. (郝长春, 孙润广, 张静, 常怡光, 牛春玲, 中国科学(B辑), 2008, 38, 1018.)
[27] Sun, R. G.; Hao, C. C.; Chang, Y. G.; Zhang, J.; Niu, C. L. Acta Chim. Sinica 2009, 67, 1808. (孙润广, 郝长春, 常怡光, 张静, 牛春玲, 化学学报, 2009, 67, 1808.)
[28] Karen, S.; Juha-Pekka, M.; Paavo, K. J. Biophys. J. 2008, 95, 2342.
[29] Domenech, O.; Sanz, F.; Montero, M. T.; Hernandez-Borrell, J. Biochim. Biophys. Acta 2006, 1758, 213.
[30] Hac-Wydro, K.; Dynarowicz-?atka, P.; Grzybowska, J.; Borowski, E. Colloids Surf. B 2005, 46, 7.
[31] Barbara, G. M.; Maria, P. Thin Solid Films 2008, 516, 8865.
[32] Ohta, Y.; Yokoyama, S.; Sakai, H.; Abe, M. Colloids Surf. B 2004, 34, 147.
[33] Katarzyna, H. W. Chem. Phys. Lipids 2010, 163, 689.
Options
文章导航

/

〈 〉