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2013 , Vol. 71 >Issue 11: 1535 - 1539

DOI: https://doi.org/10.6023/A13060678

研究论文

聚二甲基硅氧烷-聚苯乙烯复合微流控芯片室温不可逆封合法的研究

  • 胡贤巧 ,
  • 何巧红 ,
  • 白泽清 ,
  • 苏法铭 ,
  • 陈恒武
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  • 浙江大学化学系 微分析系统研究所 杭州 310058

收稿日期: 2013-06-28

  网络出版日期: 2013-08-12

基金资助

项目受国家重点基础研究发展计划(973, No. 2007CB714502)和国家自然科学基金(No. 20890020)资助.

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An Approach for Irreversible Bonding of PDMS-PS Hybrid Microfluidic Chips at Room Temperature

  • Hu Xianqiao ,
  • He Qiaohong ,
  • Bai Zeqing ,
  • Su Faming ,
  • Chen Hengwu
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  • Department of Chemistry, Institute of Microanalytical Systems, Zhejiang University, Zijin'gang Campus, Hangzhou 310058

Received date: 2013-06-28

  Online published: 2013-08-12

Supported by

Project supported by the National Basic Research Program of China (973 Program, No. 2007CB714502) and the National Natural Science Foundation of China (No. 20890020).

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摘要

研究了一种基于紫外光/臭氧(UV/O3)表面改性和硅烷化技术的聚二甲基硅氧烷(PDMS)与聚苯乙烯(PS)的不可逆封合的新方法. 首先, 用UV/O3处理PS使其表面产生羟基、羧基等极性基团; 然后用3-氨丙基三乙氧基硅烷(APTES)对UV/O3处理后的PS硅烷化, 使其表面形成氨丙基硅分子链; 再将硅烷化后的PS与拟封合的PDMS同时用UV/O3处理, 使两者表面均产生硅羟基. 最后将处理后的PDMS与PS贴合, 通过硅羟基之间的缩合实现两者的不可逆封合. 以接触角、XPS和ATR-FT-IR对封合过程进行表征. 封合的PDMS-PS复合芯片可承受大于0.5 MPa的压强. 采用该方法制备了PDMS-PS复合微流控芯片用于HeLa细胞的培养. 实验表明, HeLa细胞在PDMS-PS复合芯片通道内的生长状况大大优于在全PS芯片、略好于在全PDMS芯片内的生长状况.

关键词： 复合芯片; 聚二甲基硅氧烷; 聚苯乙烯; 不可逆封合; 细胞培养

本文引用格式

胡贤巧 , 何巧红 , 白泽清 , 苏法铭 , 陈恒武 . 聚二甲基硅氧烷-聚苯乙烯复合微流控芯片室温不可逆封合法的研究[J]. 化学学报, 2013 , 71(11) : 1535 -1539 . DOI: 10.6023/A13060678

Abstract

In some circumstances, hybrid polymer microfluidic chips composed of both elastic, gas-permeable polydimethylsiloxane (PDMS) and rigid plastics are needed. However, it is quite difficult to bond PDMS irreversibly to plastics such as polystyrene (PS). In this article, a facile method for irreversible bonding of PDMS to PS was proposed based on UV/O3-assisted surface modification in combination of surface silanization. Firstly, a PS sheet was exposed to UV/O3 to produce oxygen-containing polar moieties, such as hydroxyl and carboxylic acid, on its surface. Secondly, the UV/O3 treated PS sheet was silanized with (3-aminopropyl)triethoxysilane (APTES) via the reaction between the oxygen-containing polar moieties on the PS surface and the molecules of APTES. Thirdly, the silanized-PS sheet and the PDMS substrate with micro channel network were treated with UV/O3 again to generate silanol moieties on both surfaces. Finally, the UV/O3 treated PDMS was immediately brought intimate contact with the UV/O3 treated silanized-PS, and irreversible bonding of PDMS with PS occurred after putting the PDMS-PS complex at room temperature for 1 h through the condensation reaction between silanol moieties. Contact angle measurement, X-ray photoelectron spectroscopy, total reflection Fourier transformation infrared spectrometer were applied to characterize the surface chemistry of the PS during the UV/O3 treatment and silanization. The hybrid PDMS-PS microfludic chips prepared with the established method can bear a gas pressure higher than 0.5 MPa and a water stream at a flow rate higher than 170 μL/min (the test channels were 2.5 cm in the length, 50 μm in the width, 200 μm in the depth). A hybrid PDMS-PS microfluidic chip composed of gas-permeable PDMS substrate with channel network and excellently biocompatible PS cover sheet was fabricated for cell culture. The experimental results showed that HeLa cells cultured in the hybrid PDMS-PS microchip grew much better than those cultured in the full PS microchip, and slightly better than those in the full PDMS microchip.

Key words： hybrid microfluidic chips; polydimethylsiloxane; polystyrene; irreversible bonding; cell culture

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