纳米多孔金的单分子层门控离子输运性质研究
收稿日期: 2017-05-17
网络出版日期: 2017-09-04
基金资助
国家自然科学基金（No.21571011）和国家重点基础研究发展计划（No.2014CB931803）资助.
Monolayer-gated Ion Transport in Artificial Ion Channels Based on A Nanoporous Gold Membrane
Received date: 2017-05-17
Online published: 2017-09-04
Supported by
Project supported by the National Natural Science Foundation of China (No. 21571011) and the National Basic Research Program of China (No. 2014CB931803).
生物离子通道能够对环境刺激作出响应，有效地调节细胞内外的物质平衡，保证体内的正常生命活动.研究具有生物离子通道功能的人工离子通道对发展离子开关具有重要的意义.本工作利用电化学去合金法制备了具有三维通道结构的纳米多孔金膜，并将其应用于离子通道，研究其离子输运性质.在电场作用下，纳米多孔金由于发生极化而使通道表现出离子整流性质.在纳米多孔金表面修饰十二烷基硫醇单分子层，利用其疏水效应，通道能够阻止离子的传输，使其处于“关闭”态.溶液中的化学刺激如表面活性剂能够增强电解质溶液在通道表面的浸润，有利于离子的传输，使通道处于“打开”态.这种单分子层修饰的纳米多孔金表现出表面活性剂响应的离子开关特性.
闫娜娜 , 肖天亮 , 刘兆阅 . 纳米多孔金的单分子层门控离子输运性质研究[J]. 化学学报, 2017 , 75(9) : 873 -877 . DOI: 10.6023/A17050216
Biological ion channels that intelligently control the transport of ions or molecules through the cell membrane in response to external stimuli can maintain the balance between the extracellular and intracellular substances, which ensures the normal life activities of the organism. The development of artificial ion channels with analogous function to the biological counterparts is of great significance because of their possible applications as ion switches and sensors. In this paper, we describe a new type of artificial ion channels based on de-alloyed nanoporous gold membrane with three-dimensional nanochannels. The nanochannels were built by electrochemical etching of gold-silver alloy in concentrated nitric acid. The surface morphology and component of nanoporous gold membrane were characterized by scanning electron microscope (SEM) and energy dispersive X-ray spectrum (EDX). The ion transport properties of artificial ion channels were characterized with current-voltage curves which was measured by a picoammeter. Our results indicate that this nanoporous gold membrane demonstrates an ion rectification phenomenon because of the electrochemical polarization of gold under an electric field. Subsequently, a layer of hydrophobic molecules was assembled on the surface of nanoporous gold membrane by strong Au-thiol bonds after immersed in the solution of 1-dodecanethiol. The measurements of water contact angles (CAs) indicated that the modification of 1-dodecanethiol molecules converted the surface water CA of nanoporous gold membrane from 36.5° to 120.6°. This hydrophobic monolayer prevents the transport of water-soluble ions, which makes the channels exist in an "off" state. The stimulus of surfactant in the electrolyte is favorable for the wetting of channel surface by aqueous electrolyte, which makes the channels exist in an "on" state for water-soluble ions. Therefore, the monolayer-modified nanoporous gold membrane can serve as a surfactant-gated ion switch. Our work provides a new idea for the preparation of artificial ion channels, which can be applied for intelligently responsive artificial system.
Key words： ion channel; de-alloying; nanoporous gold; surfactant; ion switch
[1] Hille, B. Ion Channels of Excitable Membranes, Sinauer Associates, Sunderland, 2001, pp. 1～21.
[2] Tang, C.; Wang, L.; Yun, Y.; Zhang, C.; Liu, B. Acta Chim. Sinica 2011, 69, 343 (in Chinese). (唐橙橙, 王丽华, 贠延滨, 张陈淋, 刘必前, 化学学报, 2011, 69, 343.)
[3] Lu, J.; Li, J. H. Angew. Chem. Int. Ed. 2015, 54, 13576.
[4] Cooper, G. M.; Hausman, R. E. The Cell: A Molecular Approach, Sinauer Associates, Sunderland, 2000, pp. 58～65.
[5] Sigworth, F. J. Q. Rev. Biophys. 1994, 27, 1.
[6] Roux, B.; Berneche, S.; Egwolf, B.; Lev, B.; Noskov, Y.; Rowley, N.; Yu, H. J. Gen. Physiol. 2011, 137, 415.
[7] Hou, X.; Guo, W.; Jiang, L. Chem. Soc. Rev. 2011, 40, 2385.
[8] Hou, X.; Jiang, L. ACS Nano 2009, 3, 3339.
[9] Siwy, Z. S.; Howorka, S. Chem. Soc. Rev. 2010, 39, 1115.
[10] Xu, Y.; Meng, Z.; Zhai, J. Acta Chim. Sinica 2016, 74, 538 (in Chinese). (许阳蕾, 孟哲一, 翟锦, 化学学报, 2016, 74, 538.)
[11] Lan, W.; Holden, D. A.; White, H. S. J. Am. Chem. Soc. 2011, 133, 13300.
[12] White, H. S.; Bund, A. Langmuir 2008, 24, 2212.
[13] Liu, S.; Dong, Y.; Zhao, W.; Xie, X.; Ji, T.; Yin, X.; Liu, Y.; Liang, Z.; Momotenko, D.; Liang, D.; Girault, H.; Shao, Y. Anal. Chem. 2012, 84, 5565.
[14] Chen, P.; Mitsui, T.; Farmer, D. B.; Golovchenko, J.; Gordon, R. G.; Branton, D. Nano Lett. 2004, 4, 1333.
[15] Ali, M.; Yameen, B.; Cervara, J.; Ramírez, P.; Neumann, R.; Ensinger, W.; Knoll, W; Azzaroni, O. J. Am. Chem. Soc. 2010, 132, 8338.
[16] Zhang, M.; Hou, X.; Wang, J.; Tian, Y.; Xia, F.; Zhai, J.; Jiang, L. Adv. Mater. 2012, 24, 2424.
[17] Ali, M.; Ramirez, P.; Nguyen, H. Q.; Nasir, S.; Cervera, J.; Mafe, S.; Ensinger, W. ACS Nano 2012, 6, 3631.
[18] Zhou, D.; Meng, Z.; Zhang, M.; Zhai, J. Acta Chim. Sinica 2015, 73, 716 (in Chinese). (周迪, 孟哲一, 张明辉, 翟锦, 化学学报, 2015, 73, 716.)
[19] Ali, M.; Nasir, S.; Ramirez, P.; Ahmed, I.; Nguyen, Q. H.; Fruk, L.; Mafe, S.; Ensinger, W. Adv. Funct. Mater. 2012, 22, 390.
[20] Guo, W.; Xia, H.; Xia, F.; Hou, X.; Cao, L.; Wang, L.; Xue, J.; Zhang, G.; Song, Y.; Zhu, D.; Wang, Y.; Jiang, L. Chem. Phys. Chem. 2010, 11, 859.
[21] Vlassiouk, I.; Siwy, Z. Nano Lett. 2007, 7, 552.
[22] Xia, F.; Guo, W.; Hou, X.; Xue, J.; Xia, H.; Wang, L.; Song, Y.; Ji, H.; Ouyang, Q.; Wang, Y.; Jiang, L. J. Am. Chem. Soc. 2008, 130, 8345.
[23] Jog, P. V.; Gin, M. S. Org. Lett. 2008, 10, 3693.
[24] Kumar, S. K.; Hong, J. D. Langmuir 2008, 24, 4190.
[25] Yameen, B.; Ali, M.; Neumann, R.; Ensinger, W.; Knoll, W.; Azzaroni, O. Nano Lett. 2009, 9, 2788.
[26] Lin, L.; Liu, Y.; Yan, J.; Wang, X. S.; Li, J. H. Anal. Chem. 2013, 85, 334.
[27] Hou, X.; Guo, W.; Xia, F.; Nie, F.; Dong, H.; Tian, Y.; Wen, L.; Wang, L.; Cao, L.; Yang, Y.; Xue, J.; Song, Y.; Wang, Y.; Liu, D.; Jiang, L. J. Am. Chem. Soc. 2009, 131, 7800.
[28] Matsumoto, F.; Nishio, K.; Masuda, H. Adv. Mater. 2004, 16, 2105.
[29] Mara, A.; Siwy, Z.; Trautmann, C.; Wan, J.; Kamme, F. Nano Lett. 2004, 4, 497.
[30] Hu, Z. Y.; Zhang, Q. Q.; Gao, J.; Liu, Z. Y.; Zhai, J.; Jiang, L. Langmuir 2013, 29, 4806.
[31] Haque, F.; Li, J.; Wu, H.; Liang, X.; Guo, P. Nano Today 2013, 8, 56.
[32] Lin, L.; Yan, J.; Li, J. Anal. Chem. 2014, 86, 10546.
[33] Van Der Heyden, F. H.; Bonthuis, D. J.; Stein, D.; Meyer, C.; Dekker, C. Nano Lett. 2007, 7, 1022.
[34] Xie, Y.; Wang, X.; Xue, J.; Jin, K.; Chen, L.; Wang, Y. Appl. Phys. Lett. 2008, 93, 163116.
[35] Zhang, Q. Q.; Xiao, T. L.; Yan, N. N.; Liu, Z. Y.; Zhai, J.; Diao, X. G. Nano Energy 2016, 28, 188.
[36] Guo, W.; Cao, L.; Xia, J.; Nie, F.; Ma, W.; Xue, J.; Song, Y.; Zhu, D.; Wang, Y.; Jiang, L. Adv. Funct. Mater. 2010, 20, 1339.
[37] Wen, L.; Hou, X.; Tian, Y.; Zhai, J.; Jiang, L. Adv. Funct. Mater. 2010, 20, 2636.
[38] Martin, R.; Nishizawa, M.; Jirage, K.; Kang, M.; Lee, S. Adv. Mater. 2001, 13, 1351.
[39] Vlassiouk, I.; Kozel, T. R.; Siwy, Z. S. J. Am. Chem. Soc. 2009, 131, 8211.
[40] Hou, X.; Dong, H.; Zhu, D.; Jiang, L. Small 2010, 3, 361.
[41] Siwy, Z. S.; Heins, E.; Harrell, C.; Kohli, P.; Martin, R. J. Am. Chem. Soc. 2004, 126, 10850.
[42] Lee, B.; Martin, R. J. Am. Chem. Soc. 2002, 124, 11850.
[43] Nishizawa, M.; Menon, P.; Martin, R. Science 1995, 268, 700.
[44] Yang, C.; Hinkle, P.; Menestrina, J.; Vlassiouk, I.; Siwy, Z. S. J. Phys. Lett. 2016, 7, 4152.
[45] Li, X. L.; Wang, Y.; Zhai, J. Acta Chim. Sinica 2016, 74, 597 (in Chinese). (李秀林, 汪洋, 翟锦, 化学学报, 2016, 74, 597.)
[46] Ding, Y.; Kim, Y.; Erlebacher, J. Adv. Mater. 2004, 16, 1897.
[47] Xu, C.; Su, J.; Xu, X.; Liu, P.; Zhao, H.; Tian, F.; Ding, Y. J. Am. Chem. Soc. 2007, 129, 42.
[48] Erlebacher, J.; Aziz, M. J.; Karma, A.; Dimitrov, N.; Sieradzki, K. Nature 2001, 410, 450.
[49] Newman, R.; Sieradzki, K. Science 1994, 263, 1708.
[50] Polat, O.; Seker, E. J. Phys. Chem. C 2015, 119, 24812.
[51] Forty, A. J.; Durkin, P. Philos. Mag. A 1980, 42, 295.
[52] Zheng, D.; Hu, C.; Gan, T.; Dang, X.; Hu, S. Sens. Actuat. B-Chem. 2010, 148, 247.
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