基于点击化学的同步辐射X射线成像标签★
收稿日期: 2023-03-02
网络出版日期: 2023-04-12
基金资助
受国家重点研发计划(2022YFA1603600); 国家自然科学基金(22022410); 国家自然科学基金(82050005); 上海市2022年度“科技创新行动计划”基础研究领域项目(22JC1401203); 中科院青年创新促进会(2016236)
Click Chemistry-based Synchrotron X-ray Imaging Tags★
Received date: 2023-03-02
Online published: 2023-04-12
Supported by
National Key Research and Development Program of China(2022YFA1603600); National Natural Science Foundation of China(22022410); National Natural Science Foundation of China(82050005); 2022 Shanghai “Science and Technology Innovation Action Plan” Fundamental Research Project(22JC1401203); Youth Innovation Promotion Association CAS(2016236)
基于同步辐射的X射线显微成像技术具有高空间分辨率和良好的能量(元素)分辨能力, 在细胞内生物分子的识别和成像中具有很大的应用潜力. 然而, 目前已发展的与同步辐射X射线显微技术相适合的对细胞内多种生命靶标同时进行识别与成像的探针技术仍较缺乏. 本工作应用X射线具有良好的能量分辨、且元素谱间互不干扰的特点, 可控合成聚多巴胺(PDA)纳米颗粒, 在PDA纳米颗粒上修饰叠氮基并螯合金属离子, 发展了基于点击化学的同步X射线成像标签(PDA-N3-Metal), 并以30 nm的成像分辨率对该标签进行同步辐射X射线成像. 研究结果为进一步制备基于点击化学的X射线探针, 实现同时对细胞内多种生物分子的特异性识别和成像打下了良好的基础.
汤乔伟 , 蔡小青 , 殷大鹏 , 孔华庭 , 张祥志 , 张继超 , 闫庆龙 , 诸颖 , 樊春海 . 基于点击化学的同步辐射X射线成像标签★[J]. 化学学报, 2023 , 81(5) : 441 -444 . DOI: 10.6023/A23030061
One of the basic goals of cell biology is to identify multiple biological molecules within cells and understand the complex interactions between biological molecules in cellular life activities. Synchrotron-based X-ray microscopy has high spatial resolution and good energy (element) resolution, which has great application potential in the recognition and imaging of intracellular biomolecules. At present, the probes that have been developed for synchrotron-based X-ray microscopy are mainly immunostaining probes and genetic labeling probes. Immunostaining probes are prone to lead to cross-reactions due to their dependence on antigen-antibody reactions. The genetic labeling probes, based on gene coding tags, catalyze the generation of X-ray sensitive polymers. However, the polymers used to provide X-ray imaging signals have no fixed morphology. When it is applied to cell imaging, the positioning accuracy will be reduced due to the diffusion of tags in cells, which is an inherent defect of such imaging tags. In addition, there are few existing systems that can express each other independently and step by step for this type of probe. Therefore, both types of X-ray probes mentioned above are difficult to achieve simultaneous high-resolution imaging observation of multiple biological target molecules in cellular life activities. In this research paper, by using the characteristics of X-ray that has good energy resolution and does not interfere with each other between element spectra, we can synthesize polydopamine (PDA) nanoparticles in a controlled manner, modify azide groups on PDA nanoparticles and chelate metal ions, develop a click chemistry based synchronous X-ray imaging tag (PDA-N3-Metal), and conduct synchrotron radition X-ray imaging on the tag with an imaging resolution of 30 nm. The research results lay a good foundation for further preparation of X-ray probes based on click chemistry, and for realizing the specific recognition and imaging of multiple biological molecules in cells at the same time.
Key words： polydopamine; click chemistry; synchrotron-based X-ray; metal; imaging tag
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