多酸型α-葡萄糖苷酶抑制剂的抗氧化性能研究
收稿日期: 2023-06-07
网络出版日期: 2023-07-11
基金资助
国家自然科学基金(22271119)
Study on the Antioxidant Properties of Polyoxometalates α-Glucosidase Inhibitors
Received date: 2023-06-07
Online published: 2023-07-11
Supported by
National Natural Science Foundation of China(22271119)
本研究合成并表征了三类(母体、过渡金属取代的磷钼酸及不同钒个数取代的磷钼酸共11个)Dawson型磷钼酸, 通过对2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS)自由基、羟基自由基、1,1-二苯基-2-三硝基苯肼(DPPH)自由基及超氧阴离子自由基的清除效果考察11种化合物的体外抗氧化性能. 通过噻唑蓝(MTT)法检测细胞存活率, 结合体外抗氧化及细胞毒性实验结果, 对H6[P2Mo18O62], H7[P2Mo17VO62]和H8[P2Mo16V2O62]进行细胞抗氧化研究, 检测其细胞内总抗氧化能力和对细胞超氧化物歧化酶(SOD)活性的影响. 将多酸与α-葡萄糖苷酶进行分子对接, 探究它们之间的相互作用机制. 结果表明11种化合物均具有较好的抗氧化性能. 分子对接结果显示多酸主要通过氢键和范德华力与活性中心的氨基酸残基进行结合. 其中, H6[P2Mo18O62], H8[P2Mo17Fe(OH2)O61], H8[P2Mo17Ni(OH2)O61], H7[P2Mo17VO62]和H8[P2Mo16V2O62]表现优异, 有望成为兼具α-葡萄糖苷酶抑制效用的抗氧化剂备选材料.
李瑶 , 陈丙年 , 罗丹 , 雷珊 , 王力 . 多酸型α-葡萄糖苷酶抑制剂的抗氧化性能研究[J]. 化学学报, 2023 , 81(10) : 1318 -1326 . DOI: 10.6023/A23060276
Three types of Dawson type phosphomolybdate were synthesized and the in vitro antioxidant properties of the 11 compounds were investigated by the scavenging effect of different concentrations of polyoxometalates (0~50 mg/mL with distilled water and Vitamin C (VC) as positive control, three parallel experiments were set up for each group) on 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radicals, hydroxyl radicals, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals and superoxide anion radicals. The cytotoxicity of the compounds was assayed by methyl thiazolyl tetrazolium (MTT) assay and combined with the results of in vitro antioxidant and cytotoxicity assays, cellular antioxidant studies were performed on H6[P2Mo18O62], H7[P2Mo17VO62] and H8[P2Mo16V2O62] using HepG2 cells as a model. The total intracellular antioxidant capacity was measured using the ABTS method: 10 μL of the sample/standard solution and 200 μL of the ABTS working solution were mixed in a 96-well plate for 5 min at room temperature, and the absorbance of the sample was measured at 734 nm using a multifunctional enzyme marker. WST-1 assay for cellular superoxide dismutase (SOD) activity: The samples to be tested were diluted to different concentrations and the absorbance values were measured at 450 nm using a multifunctional enzyme marker. The SOD inhibition rate of the sample to be tested was calculated and a sample concentration of 40%~60% inhibition was selected for the experiment. Cellular antioxidant assays showed that H6[P2Mo18O62] had a total cellular antioxidant capacity comparable to that of VC at higher concentrations (25 μmol/L and above); it enhanced the activity of cellular superoxide dismutase (SOD) and was more active than VC. We investigated the interaction mechanisms of molecular docking between polyoxometalates and α-glucosidase. The results showed that all 11 compounds had good antioxidant properties, and molecular docking showed that the binding inhibition of the polyoxometalates to the amino acid residues in the active centre was reversible mainly through hydrogen bonding and van der Waals forces, and that the docking fraction was negative and the reaction could proceed spontaneously, but the types of amino acids involved varied. Among them, H6[P2Mo18O62], H8[P2Mo17Fe(OH2)O61], H8[P2Mo17Ni(OH2)O61], H7[P2Mo17VO62] and H8[P2Mo16V2O62] performed well and could be alternative materials for antioxidants with both α-glucosidase inhibiting effect.
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