Microbiological hydroxylation of 16-dehydroprogesterono(1) with Absidia orchidis afforded 15β-hydroxy 16-dehydroprogesterone(2),7β,15β-dihydroxy-l6-dehydro- progestrone(S) and 6β,15β-dihydroxy 16-dehydroprogesterone(4).Compound(3) was the major product.The result was different from that of hydroxylation with Rhizopus nigricans which afforded only the 11a-hydroxy derivative with the saturation of C₁₆ double bond to give the unnatural 17α-configuration.The structure of compounds 2-4 have been characterized through chemical trans-formations and spectroscopic evidences.