The title compound has been prepared from the reaction from the reaction of fluorescein and benzoyl chloride. its crystaland molecular structure is determined by singly crystal X-ray diffraction.its crystal is monoclinicspace group P21/C with lattice parameters a=0.8459(2), b=1.8221(5), c=1.7491(8)nm, β=103.79(3)°, V=2.618nm3, Z=4. decomposition of fluorescein drivatives by lipase and saponification experiments indicate that hydrolysis rate of fluorescein dibenzoate of fluorescein.these features are explained according to the steric hindrance and conjugation effect of the ester group.

Key words: CRYSTAL STRUCTURE, HYDROLYSIS, LUCIFERIN, BENZOYL CHLORIDE