A nanoelectrospray interface was set up for hyphenating with orthogonal injection time-of-flight mass spectrometer. Low detection limit (about 0.8 ～1.2 zmol) and low sample consumption (about 0.02 amol) per spectrum was obtained by injecting horse heart cytochrome c into the mass sepectrometer with a nanoelectrospray interface at a flow rate of 30 nL/min. As smaller droplet is formed in nanoelectrospray process, the desolvation efficiency and the ionization efficiency could be enhanced and thus may facilitate naalysis of oligosaccharides. A better signal was obtained in analyzing SBE-β-CD by the nanoelectrospray interface compared with a microspray interface. In addtion, in-source CID experiment of agiotensin I and trypsin enzymatic peptides map of cytochrome c were achieved by nanoelectrospray interface, respectively. The results showed that all but one ion in cytochrome c was achieved clearly in nanoelectrospray spectrum.

Key words: TIME-OFF FLIGHT MASS SPECTROMETRY, CYTOCHROME, POLYPEPTIDE