A novel chemiluminescence reaction detector was developed for the simultaneous determination of isoniazid (INH) and rifampin (RFP), which are the two most active first-line drugs for treatment of tuberculosis, separated by HPLC based on the direct hexacyanoferrate ( HI ) chemiluminescence reaction by isoniazid and rifampin in sodium hydroxide medium. The separation was carried out on Kromasil RP-C- 18column (150 mmx 4.6 mm i.d., 5 jum) at 35 °C . The mobile phase consisted of a F(methanol) V(water) = 60-40 solution. At a flow-rate of 0.8 mL/min, the total run time was ~ 6 min. Parameters affecting CL detection, such as CL reaction reagent [K3Fe(CN)

Key words: Isoniazid, Rifampin, HPLC, CHEMILUMINESCENCE ANALYSIS, POTASSIUM FERRICYANIDE, ANTITUBERCULAR AGENTS