A novel homogenous fluorogenic probe, TaqMan-molecular beacon (TaqMan-MB) has been developed from TaqMan and molecular beacons, which combines the hairpin structure of molecular beacons and the cleavage mechanism of TaqMan probes and has much better detection effects than TaqMan or molecular beacons alone. Combing with a real-time PCR instrument, TaqMan-MB presents a promising approach for quantitation of target genes.

Key words: GENE, FLUORESCENCE, PROBES, POLYMERIZATION ENZYME