Micro-plate Magnetic Chemiluminescence Enzyme Immunoassay for Rapid, Sensitive Determination of Human Chorionic Gonadotropin (HCG)

Acta Chimica Sinica ›› 2004, Vol. 62 ›› Issue (1): 71-77. Previous Articles Next Articles

微板式磁化学发光酶免疫分析法对人绒毛膜促性腺激素(HCG)的灵敏快速测定

赵利霞, 林金明, 屈锋

中国科学院生态环境研究中心, 北京, 100085

投稿日期:2003-05-07 修回日期:2003-08-31 发布日期:2014-01-26
通讯作者: 林金明 E-mail::E-mail:jmlin@mail.rcees.ac.cn
基金资助:
国家杰出青年科学基金(No.20125514)资助项目.

Micro-plate Magnetic Chemiluminescence Enzyme Immunoassay for Rapid, Sensitive Determination of Human Chorionic Gonadotropin (HCG)

ZHAO Li-Xia, LIN Jin-Ming, QU Feng

Research Center for Eco-environmental Sciences, Chinese Academy of Sciences, Beijing 100085

Received:2003-05-07 Revised:2003-08-31 Published:2014-01-26

Abstract

A micro-plate magnetic chemiluminescence enzyme immunoassay for the determination of human chorionic gonadotropin (HCG) based on the improvement in the method of the magnetic immunoenzyme spectrophotometric assay was established. The enzyme alkaline phosphatase (ALP) was used as labeled reagent, which reacted with the chemiluminescence reagent of 3-(2′-spiroadamantane)-4-methoxy-4-(3″-phosphoryloxy)phenyl-1,2-dioxetane (AMPPD) in buffer solution. The sensitivity of the present chemiluminescent method was 13 times higher than that of spectrophotometry. The linear range was 0.15～500 mIU/mL HCG; the within run and between run CVs were both below 18%. This method was applied to detect HCG in serum and the percent recovery was in the range of 80%～116%. Compared with other chemiluminescence immunoassays, the correlation coefficient was 0.965. It was firstly reported to use enzyme immunoassay for the detection of HCG in saliva, which will break a new way for establishing a non-invasive method.

Key words: chemiluminescence, human chorionic gonadotropin(HCG), enzyme immunoassay, saliva

Cite this article

ZHAO Li-Xia, LIN Jin-Ming, QU Feng . Micro-plate Magnetic Chemiluminescence Enzyme Immunoassay for Rapid, Sensitive Determination of Human Chorionic Gonadotropin (HCG)[J]. Acta Chimica Sinica, 2004, 62(1): 71-77.

Export EndNote|Reference Manager|ProCite|BibTeX|RefWorks

share this article

References

[1] Swaminathon, N. Bahl. OP. Fed. Proc. 1970, 29, 599.
[2] Xu, R.-Q. Zhonghua Fuchanke Zazhi 1981, 16, 29 (in Chinese).(徐仁淇, 中华妇产科杂志, 1981, 16, 29.)
[3] Kokado, A.; Arakawa, H.; Maeda, M. Anal. Chim. Acta 2000, 407, 119.
[4] He, Z.-H.; Gao, N.; Jin, W.-R. J. Chromatogr. B 2003, 784, 343.
[5] Nakamura, N.; Lim, T.-K.; Jeong, J.-M.; Matsunaga, T. Anal. Chim. Acta 2001, 439, 125.
[6] Zhang, F.; Li, Q.-G.; Li, Z.-Q.; Chen, J. Chin. J. Anal. Chem. 1993, 21, 698 (in Chinese).(张帆, 李庆阁, 李兆强, 陈静, 分析化学, 1993, 21, 698.)
[7] Vankrieken, L.; Hertogh, R. D. Clin. Chem. 1995, 41, 36.
[8] Lin, J.-M.; Tsuji, A.; Maeda, M. Anal. Chim. Acta 1997, 339, 139.
[9] Han, P.-Z. Foreign Medicine*The Fascicule of Radiomedicine and Nuclearmedicine 2000, 24, 196 (in Chinese).(韩佩珍, 国外医学*放射医学核医学分册, 2000, 24, 196.)
[10] Korest, J.-C.; Masse, J.; Lane. A. Clin. Biochem. 1998, 31, 81.
[11] Rattle, S. J.; Purnell, D. R.; Williams, P. I. M.; Siddle, K.; Forrest, G. C. Clin. Chem. 1984, 30, 1457.
[12] Filaire, E.; Bernain, X.; Sagnol, M.; Lac, G. Eur. J. Appl. Physiol. 2001, 86, 179.
[13] Morineau, G.; Boudi, A.; Barka, A. Gourmelen, M.; Degeilh, F.; Hardy, N.; Al-Halnak, A.; Soliman, H.; Gosling, J. P.; Julien, R.; Brerault, J.-L.; Boudou, P.; Aubert, P.; Villette, J.-M.; Pruna, A.; Galons, H.; Fiet, J. Clin. Chem. 1997, 43, 1397.
[14] Cook, N. J.; Scahefer, A. L.; Lepage, P.; Jones, S. D. M. J. Agric. Food Chem. 1997, 45, 395.
[15] Yao, J.-K.; Moss, H. B.; Kirillova, G. P. Clin. Biochem. 1998, 31, 187.
[16] Putignano, P.; Dubin, A.; Toja, P.; Invitti, C.; Bonfanti, S.; Redaelli, G.; Zappulli, D.; Cavagnini, F. Eur. J. Endocrinol. 2001, 145, 165.
[17] Anfossi, L.; Tozzi, C.; Giovannoli, C.; Baggiani, C.; Giraudi, G. Anal. Chim. Acta 2002, 468, 315.
[18] Lac, G. Pathol. Biol. 2001, 49, 660.
[19] Mao, H.-Y.; Zhao, X. Zunyi Yixueyuan Xuebao 1997, 20, 67 (in Chinese).(苗海玉, 赵宪, 遵义医学院学报, 1997, 20, 67.)
[20] Deacon, J. K.; Thomson, A. M.; Page, A. L.; Stops, J. E.; Roberts, P. R.; Whiteley, S. C.; Attridge, J. W.; Love, C. A.; Robinson, G. A.; Davidson, G. P. Biosen. Bioelectron. 1991, 6, 193.
[21] Xie, S.-W.; Yang, G.-Z.; Xu, Y.-P. Clinical Immunology Technique, Shanghai Science and Technology Press, Shanghai, 1982, p. 21 (in Chinese).(谢少文, 杨桂贞, 许以平, 临床免疫技术, 上海科学技术出版社, 上海, 1982, p. 21).

微板式磁化学发光酶免疫分析法对人绒毛膜促性腺激素(HCG)的灵敏快速测定

Micro-plate Magnetic Chemiluminescence Enzyme Immunoassay for Rapid, Sensitive Determination of Human Chorionic Gonadotropin (HCG)

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	Jin Xin, Wang XiaoYing. Progress in Analysis and Detection of Salivary Tumor Biomarkers Associated with Oral Cancer [J]. Acta Chim. Sinica, 2019, 77(4): 340-350.
[2]	Zhang Zhaoxiang, Liu Yujie, Wang Qi, Wang Jingjing. Capillary Electrophoresis and Quantum Dot Electrochemiluminescence by Micellar Reversed Sweeping [J]. Acta Chim. Sinica, 2019, 77(2): 179-183.
[3]	Zhu Benzhan, Shao Bo, Mao Li, Gao Huiying. Intrinsic Chemiluminescence Production during Environmentally-friendly Advanced Oxidation of Halogenated Aromatics and Its Applications [J]. Acta Chim. Sinica, 2016, 74(7): 557-564.
[4]	Li Yuan, Liu Wenna, Zheng Xingwang. Investigation into Electrogenerated Chemiluminescence Behavior of Tris(bipyridine)ruthenium(II)/silica Nanoparticles Electrocatalyzed by Cu(tris(hydroxymethyl)aminomethane)₄²⁺ Complex [J]. Acta Chim. Sinica, 2015, 73(7): 749-754.
[5]	Wang Dabin, Zhao Lixia, Guo Lianghong, Zhang Hui, Wan Bin, Yang Yu. Online Quantification of O₂^·- and H₂O₂ and Their Formation Kinetics in Ultraviolet (UV)-Irradiated Nano-TiO₂ Suspensions by Continuous Flow Chemiluminescence [J]. Acta Chim. Sinica, 2015, 73(5): 388-394.
[6]	Zhang Lihong, Guo Zhihui. Study on the Inhibition Effect of Carbon Quantum Dots on Luminol Electrochemiluminescence System and Its Application in DNA Analysis [J]. Acta Chimica Sinica, 2013, 71(04): 644-648.
[7]	Wei Wei, Zhang Quan, Zheng Xingwang. Synthesis of Chitosan/Fe₃O₄/SiO₂ Nanocomposites and Investigation into Their Catalysis Properties [J]. Acta Chimica Sinica, 2013, 71(03): 387-391.
[8]	Tong Bihai, Mei Qunbo, Li Zhiwen, Dong Yongping, Zhang Qianfeng. Investigation on the Electrochemiluminescence Properties of a Series of Cyclometalated Iridium(III) Complexes Based on 2-Phenylquinoline Derivatives [J]. Acta Chimica Sinica, 2012, 70(23): 2451-2456.
[9]	Yang Fan, Wang Lingli, Guo Zhihui. Study on the Influence of Carbon Quantum Dots on Electrogenerated Chemiluminescence Energy Transfer Sensing System of DNA [J]. Acta Chimica Sinica, 2012, 70(11): 1283-1288.
[10]	Du Jianxiu, Wang Hong. Chemiluminescence of Nano-Colloidal MnO₂ with Formaldehyde and Its Analytical Application [J]. Acta Chimica Sinica, 2012, 0(05): 537-543 .
[11]	CHEN Xiao-Hui, DU Jian-Xiu. Chemiluminescence of Gold Nanoparticles-catalyzed Luminol-isoniazid Reaction and Its Analytical Application [J]. Acta Chimica Sinica, 2011, 69(06): 745-751.
[12]	ZHOU Kao-Wen, GU Xue-Mei, CUI Xiao-Chao, CHEN Xiao-Cui. Determination of Metoclopramide in Dog Plasma by Capillary Electrophoresis with Electrochemiluminescence [J]. Acta Chimica Sinica, 2011, 69(02): 221-225.
[13]	LI Zhi-Xiang, HE Lei, HE Nong-Ti, SHI Zhi-Yang, HONG Hua, LI Song, LIU Hong-Na, DAI E-Bin. An Applied Approach in Detecting E. coli O157:H7 Using Immunological Method Based on Chemiluminescence and Magnetic Nanoparticles [J]. Acta Chimica Sinica, 2010, 68(03): 251-256.
[14]	LI Zhi-Yong, XIAO Qi, YING Xi-Tang, LI Zhen-Jia, LIN Jin-Meng. Micro-plate Magnetic Chemiluminescence Immunoassay of Carbohydrate Antigen 125 in Serum [J]. Acta Chimica Sinica, 2010, 68(02): 162-168.
[15]	ZHANG Zhu-Jun, CHANG Beng-Beng. A Novel Chemiluminescence Array Sensor Based on Molecularly Imprinted Polymer [J]. Acta Chimica Sinica, 2009, 67(23): 2727-2731.