Acta Chimica Sinica ›› 2005, Vol. 63 ›› Issue (5): 407-410. Previous Articles     Next Articles

Original Articles

基于磁性微球的无标记化学发光端粒传感新技术

曹志娟1,刘彩云1,夏鹰2,甲菲雅亮3,卢建忠*1   

  1. (1复旦大学药学院 上海 200032)
    (2复旦大学华山医院神经外科 上海 200032)
    (3长崎大学药学院 日本)
  • 投稿日期:2004-06-15 修回日期:2004-11-10 发布日期:2010-12-10
  • 通讯作者: 卢建忠

Magnetic Bead-based Label-free Chemiluminescence Detection of Specific DNA Sequence-Telomere

CAO Zhi-Juan1, LIU Cai-Yun1, XIA Ying2, MASAAKI Kai3, LU Jian-Zhong*,1   

  1. (1 School of Pharmacy, Fudan University, Shanghai 200032)
    (2 Department of Neurosurgery, Huashan Hospital, Shanghai 200032)
    (3 School of Pharmacy, Nagasaki University, Nagasaki, Japan)
  • Received:2004-06-15 Revised:2004-11-10 Published:2010-12-10
  • Contact: LU Jian-Zhong

The label-free DNA sensing techniques are currently receiving considerable attention for the determination of pathogenic genes and the diagnosis of genetic disease, etc. Based on magnetic separation and preconcentration, a novel label-free chemiluminescence (CL) detection protocol was presented as exemplified by the successful determination of specific DNA sequence—telomere. This method was composed of three steps: (1) the dT20 arm modified in the magnetic beads was hybridized with the dA20-modified capture DNA; (2) the first step was followed by the second hybridization of the bead-captured probe with telomere; (3) the amount of telomere was determined by the guanine CL reaction with 3,4,5-trimethoxyphenylglyoxal (TMPG). The results showed that this simple and sensitive protocol was much suitable for the determination of specific DNA sequence such as telomere with a good linear correlation in the concentration range of 5×10-9~1×10-7 mol/L (R2=0.9918).

Key words: specific sequence DNA, telomere, label-free, chemiluminescence, magnetic bead