HPLC Separation of 1-Phenyl-3-methyl-5-pyrazolone Derivatized Oligosaccharides and Its Matrix-assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Identification

Acta Chimica Sinica ›› 2007, Vol. 65 ›› Issue (23): 2761-2764. Previous Articles Next Articles

1-苯基-3-甲基-5-吡唑啉酮(PMP)柱前衍生化寡糖链的HPLC分离及其激光解吸电离质谱分析

王仲孚, 张英, 林雪, 黄琳娟*

(西北大学生命科学学院西部资源生物与现代生物技术省部共建教育部重点实验室
陕西省生物技术重点实验室西安 710069)

投稿日期:2007-04-19 修回日期:2007-06-22 发布日期:2007-12-14
通讯作者: 黄琳娟

HPLC Separation of 1-Phenyl-3-methyl-5-pyrazolone Derivatized Oligosaccharides and Its Matrix-assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Identification

WANG Zhong-Fu; ZHANG Ying; LIN Xue; HUANG Lin-Juan*

(Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Shaanxi Provincial Key Laboratory of Biotechnology, Life Science College, Northwest University, Xi'an 710069)

Received:2007-04-19 Revised:2007-06-22 Published:2007-12-14
Contact: HUANG Lin-Juan

Abstract

A modified 1-phenyl-3-methyl-5-pyrazolone (PMP) pre-column derivatization procedure has been applied to oligosaccharide separation and analysis by reversed-phase high performance liquid chromatography (RP-HPLC) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). The traditional derivatization procedure of oligosaccharides with PMP has been simplified using liquid ammonia instead of sodium hydroxide aqueous solution as a base medium. The derivatives could be directly analyzed by MALDI-TOF-MS without a desalting procedure. The oligosaccharide-PMP derivative mixtures have been well separated and prepared by RP-HPLC under the optimized conditions using UV detection.

Key words: HPLC, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, 1-phenyl-3- methyl-5-pyrazolone, derivatization, glycan

Cite this article

WANG Zhong-Fu; ZHANG Ying; LIN Xue; HUANG Lin-Juan*. HPLC Separation of 1-Phenyl-3-methyl-5-pyrazolone Derivatized Oligosaccharides and Its Matrix-assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Identification[J]. Acta Chimica Sinica, 2007, 65(23): 2761-2764.

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1-苯基-3-甲基-5-吡唑啉酮(PMP)柱前衍生化寡糖链的HPLC分离及其激光解吸电离质谱分析

HPLC Separation of 1-Phenyl-3-methyl-5-pyrazolone Derivatized Oligosaccharides and Its Matrix-assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Identification

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