Preparation of Glycol-Fosfomycin Modified Zirconia Chromatography Stationary Phases and Its Application to the Separation of Proteins

Acta Chimica Sinica ›› 2009, Vol. 67 ›› Issue (22): 2619-2623. Previous Articles Next Articles

Full Papers

乙二醇-磷霉素改性氧化锆色谱固定相的制备及其在蛋白质分离中的应用

张淑琼^*,a,b，邹凤平^c，李烃^b

(a长江师范学院化学及环境科学系涪陵 408100) (b武汉大学化学与分子科学学院武汉 430072) (c中国地质大学材料科学与化学工程学院武汉 430074)

投稿日期:2008-10-24 修回日期:2009-05-10 发布日期:2009-07-23
通讯作者: 张淑琼 E-mail:hezhangshuqiong@163.com
基金资助:
教育部“新世纪优秀人才支持计划”基金（NCET-05-0616）;国家杰出青年科学基金（no.20625516）

Preparation of Glycol-Fosfomycin Modified Zirconia Chromatography Stationary Phases and Its Application to the Separation of Proteins

Zhang, Shuqiong^*,a,b，Zou, Fengping^c，Li, Ting^b

(^a Department of Chemistry and Environment Science, Changjiang Teachers College, Chongqing 408100) (^b College of Chemistry and Molecular Science, Wuhan University, Wuhan 430072) (^c Material Science and Chemical Engineering, China University of Geosciences, Wuhan 430074)

Received:2008-10-24 Revised:2009-05-10 Published:2009-07-23

Abstract

A novel glycol-fosfomycin modified zirconia (G-F-ZrO2) stationary phase was prepared and characterized by elemental analysis, FT-IR and N2 adsorption. Its chromatographic performance was evaluated using four standard proteins including lysozyme (Lys), ribonuclease-A (RNase-A), a-chymotrypsin (α-Chy) and Cytochrome C (Cyt-C) as probes, and the effects of salt concentration, pH, salt type in eluents and temperature on retention behavior of proteins were investigated. The results indicate that the stationary phase behaves mainly as a hydrophobic interaction chromatographic packing with salt concentration in the mobile phase above 1.0 mol•L－1. At the same time, the retention mechanism between protein and G-F-ZrO2 stationary phase was also discussed.

Key words: protein, glycol, fosfomycin, ZrO2, hydrophobic interaction chromatography

Cite this article

ZHANG Chu-Qiong, JU Feng-Beng, LI Jing. Preparation of Glycol-Fosfomycin Modified Zirconia Chromatography Stationary Phases and Its Application to the Separation of Proteins[J]. Acta Chimica Sinica, 2009, 67(22): 2619-2623.

Export EndNote|Reference Manager|ProCite|BibTeX|RefWorks

[1]	Ruxin Zeng, Peng R. Chen. RNA-Binding Proteome Analysis and Functional Explorations^★ [J]. Acta Chimica Sinica, 2024, 82(1): 53-61.
[2]	Jiao Kong, Lin Du, Xiangyang Li, Jidong Zhu, Ya-Qiu Long. Small Molecule Degraders Targeting the SHP2^E76A Mutant Effectively Inhibiting the Proliferation of Wild-type and Mutant SHP2 Dependent Tumor Cells [J]. Acta Chimica Sinica, 2023, 81(9): 1120-1128.
[3]	Chaofan Ma, Wei Xu, Wei Liu, Changhui Xu, Jingjie Sha. Proactive Manipulation Techniques for Protein Transport at Confined Nanoscale [J]. Acta Chimica Sinica, 2023, 81(7): 857-868.
[4]	Xueyang Yin, Kai Gu, Zhengzhong Shao. Preparation of the Protein/Polyphenylboronic Acid Nanospheres for Drug Loading and Unloading [J]. Acta Chimica Sinica, 2023, 81(2): 116-123.
[5]	Chang Li, Zhendong Zheng, Jiangnan Zheng, Ruijun Tian. Glycoprotein Identification using Cleavable Bifunctional Probes^★ [J]. Acta Chimica Sinica, 2023, 81(12): 1673-1680.
[6]	Zhenhong Yang, Xiaojuan Gan, Shuzhe Wang, Junyuan Duan, Tianyou Zhai, Youwen Liu. Preparation of Metallic Ni₃N Nanoparticles and Its Electrooxidation Performance for Ethylene Glycol^★ [J]. Acta Chimica Sinica, 2023, 81(11): 1471-1477.
[7]	Chengyu Fu, Xingyu Zhou, Peng Yang. Surface Functionalization Based on Protein Amyloid-like Aggregation^★ [J]. Acta Chimica Sinica, 2023, 81(11): 1566-1576.
[8]	Yuewen Zhong, Xining Qian, Chao Ma, Kai Liu, Hongjie Zhang. Rare Earth Biological Manufacturing and High Value-added Material Application^★ [J]. Acta Chimica Sinica, 2023, 81(11): 1624-1632.
[9]	Yuwan Chen, Wen Zhou, Xinwei Li, Kaiguang Yang, Zhen Liang, Lihua Zhang, Yukui Zhang. Research Progress of Protein-Protein Interaction Based on Liquid Chromatography Mass Spectrometry^※ [J]. Acta Chimica Sinica, 2022, 80(6): 817-826.
[10]	Chaofeng Wang, Guodong Zheng, Yue Wang, Huijia Song, Xiaoyi Chen, Ruixia Gao. Preparation of Controllable Non-covalent Functionalized Carbon Nanotubes with Metalloporphyrin-Sn Network and Application to Protein Adsorption [J]. Acta Chimica Sinica, 2022, 80(2): 126-132.
[11]	Xia Zheng, Jianting Liu, Zhang Liu, Jianjun Wang. Bio-inspired Ice-controlling Materials for Cryopreservation of Cells and Tissues [J]. Acta Chimica Sinica, 2021, 79(6): 729-741.
[12]	Haohao Fu, Haochuan Chen, Hong Zhang, Xueguang Shao, Wensheng Cai. Accurate Estimation of Protein-ligand Binding Free Energies Based on Geometric Restraints [J]. Acta Chimica Sinica, 2021, 79(4): 472-480.
[13]	Feng Wu, Qianqian Su, Lele Zhou, Pengfei Xu, Ao Dong, Weiping Qian. A Novel Protein Corona Characterization based on the Reflectometric Interference Spectroscopy with Silica Colloidal Crystal Films [J]. Acta Chimica Sinica, 2021, 79(3): 338-343.
[14]	Hui He, Lingli Zhou, Zhen Liu. Advances in Protein Biomarker Assay via the Combination of Molecular Imprinting and Surface-enhanced Raman Scattering [J]. Acta Chimica Sinica, 2021, 79(1): 45-57.
[15]	Zhang Pengcheng, Guo Jia, Zhu Gen, Fang Wenyu, Tang Qianyuan, Bao Lei, Kang Wenbin. Monte Carlo Simulations of Composition-Related Structural Transition of Disordered Peptides: The Case Study of Random Peptides Composed of Lysine, Glutamic Acids and Isoleucine [J]. Acta Chimica Sinica, 2020, 78(9): 994-1000.

乙二醇-磷霉素改性氧化锆色谱固定相的制备及其在蛋白质分离中的应用

Preparation of Glycol-Fosfomycin Modified Zirconia Chromatography Stationary Phases and Its Application to the Separation of Proteins

PDF

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments