H-ras Gene Mutation Detection of Gastric Cancer by Restriction Fragment Length Polymorphism with Capillary Electrophoresis

Acta Chimica Sinica ›› 2009, Vol. 67 ›› Issue (4): 323-328. Previous Articles Next Articles

Original Articles

毛细管电泳-限制性片段长度多态性分析检测胃癌H-ras基因点突变

刘圆圆a,b,c 王荣*,a 贾正平a 郭志强a 辛晓婷a,c
谢华a 马骏a 王娟a 李文斌a

(a兰州军区兰州总医院临床药理基地兰州 730050)
(b甘肃省红十字血液中心兰州 730046)
(c兰州大学生命科学学院兰州 730000)

投稿日期:2008-01-02 修回日期:2008-08-27 发布日期:2009-02-28
通讯作者: 王荣

H-ras Gene Mutation Detection of Gastric Cancer by Restriction Fragment Length Polymorphism with Capillary Electrophoresis

Liu, Yuanyuan ^a,b,c Wang, Rong ^*,a Jis, Zhengping ^a Guo, Zhiqiang ^a
Xin, Xiaoting ^a,c Xie, Hua ^a Ma, Jun ^a Wang, Juan ^a Li, Wenbin ^a

(a Department of Pharmacy, Lanzhou General Hospital of PLA, Lanzhou 730050)
(b Gansu Red Cross Blood Center, Lanzhou 730046)
(c Life Science College, Lanzhou University, Lanzhou 730000)

Received:2008-01-02 Revised:2008-08-27 Published:2009-02-28
Contact: WANG Rong

Abstract

To establish the high performance detection method for separating products of restrictive enzyme DNA fragments and diagnosing mutation gene of gastric cancer by restriction fragment length polymorphism with capillary electrophoresis, methyl cellulose was used to separate pUC19 DNA/Msp I (Hpa II) fragments through capillary electrophoresis as sieving matrix. The effects of the concentration of methyl cellulose solution, running pH, running temperature and electric field strength on dsDNA (＜600 bp) analysis were investigated in the experiment. The optimum separation condition was obtained and applied to mutation detection of H-ras gene at codon 12 of 59 gastric cancer tissues. Methyl cellulose is an excellent medium and it will make diagnosis of genetic disease rapidly, exactly, conveniently and sensitively.

Key words: capillary electrophoresis, methyl cellulose, restriction fragment length polymorphism, H-ras, gene mutation

Cite this article

WANG Rong. H-ras Gene Mutation Detection of Gastric Cancer by Restriction Fragment Length Polymorphism with Capillary Electrophoresis[J]. Acta Chimica Sinica, 2009, 67(4): 323-328.

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毛细管电泳-限制性片段长度多态性分析检测胃癌H-ras基因点突变

H-ras Gene Mutation Detection of Gastric Cancer by Restriction Fragment Length Polymorphism with Capillary Electrophoresis

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