Acta Chimica Sinica ›› 2010, Vol. 68 ›› Issue (01): 50-54. Previous Articles     Next Articles

Full Papers

环境气氛对微量热安瓿法测定细菌生长速率常数的干扰

屈啸声,林瑞森*,方文军   

  1. (浙江大学化学系 杭州 310027)
  • 投稿日期:2008-11-25 修回日期:2009-03-23 发布日期:2010-01-20
  • 通讯作者: 方文军 E-mail:fwjun@zju.edu.cn
  • 基金资助:

    国家自然科学基金 (No. 20673098)资助项目

Disturbance of Environmental Atmosphere on the Growth Rate Constants of E. coli Determined by Microcalorimetry Ampoule Method

Qu Xiaosheng, Lin Ruisen*, Fang Wenjun   

  1. (Department of Chemistry, Zhejiang University, Hangzhou 310027)
  • Received:2008-11-25 Revised:2009-03-23 Published:2010-01-20
  • Contact: FANG Wen-Jun E-mail:fwjun@zju.edu.cn

A stage-like appearance occurred in the first exponential phase of several bacteria's thermogenic curves determined by a microcalorimetry ampoule method, and the growth rate constants of these stages were very different, which has not been found before in a stop-flow method and disturbs the precise antibacterial activity evaluation. In order to find the possible reasons, the growth of E. coli under different environmental atmosphere as well as the condition similar to the stop-flow method was determined and analyzed. The environmental atmosphere is mainly responsible for this variation. A criterion was recommended for the selection of growth rate constant under various atmosphere when determined by ampoule method.

Key words: microcalorimetry, ampoule method, E. coli, growth rate constant, environmental atmosphere