A novel electrochemical sensor for the detection of tolazoline based on molecularly imprinted polymer of electropolymerized o-aminothiophenol onto a gold electrode was constructed. Its response to tolazoline was evaluated by differential pulse voltammmograms (DPV). Under the optimized conditions, the response current of the proposed sensor decreased linearly in two concentration ranges of tolazoline from 0.4 to 5 μg•mL^－1 (r＝0.9986) and from 5 to 120 μg•mL^－1 (r＝0.9953) with a detection limit of 0.18 μg•mL^－1. The RSD (relative standard deviation) was about 2.47% and the response time was closed to 3 min. Moreover, the studied imprinted sensor exhibited high selectivity and long-term stability, and was successfully applied to the determination of tolazoline in human urine samples.

Key words: electrochemical sensor, molecular imprinting technique, electropolymerization film, tolazoline