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Acta Chimica Sinica ›› 2010, Vol. 68 ›› Issue (10): 982-988. Previous Articles     Next Articles

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基于离子液体共溶剂效应的氯过氧化物酶催化氧化邻苯二胺的研究

聂艳艳1，蒋育澄*,1,2，胡满成1,2，李淑妮1,2，翟全国1,2   

  1. (1陕西师范大学化学与材料科学学院 西安 710062)
    (2陕西师范大学大分子科学陕西省重点实验室 西安 710062)
  • 投稿日期:2009-10-10 修回日期:2009-12-29 发布日期:2010-01-21
  • 通讯作者: 蒋育澄 E-mail:jyc@snnu.edu.cn
  • 基金资助:

    国家自然科学基金(No. 20876094)资助项目

Studies on the Oxidation of σ-Phenylenediamine Catalyzed by Chloroperoxidase in the Presence of an Ionic Liquid as Co-solvent

Nie Yanyan1 Jiang Yucheng*,1,2 Hu Mancheng1,2 Li Shuni1,2 Zhai Quanguo1,2   

  1. (1 School of Chemistry and Materials Science, Shaanxi Normal University, Xi an 710062)
    (2 Key Laboratory of Macromolecular Science of Shaanxi Province, Shaanxi Normal University, Xi an 710062)
  • Received:2009-10-10 Revised:2009-12-29 Published:2010-01-21

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The oxidation of σ-phenylenediamine (OPD) catalyzed by chloroperoxidase (CPO) in the presence of an ionic liquids (ILS) as co-solvent was studied in this work. Identifications by UV-Vis, FT-IR and 13C and 1H NMR spectrum show that the product is 2,3-diaminophenazine (DAP). This ensures the application of (OPD-H2O2-CPO) system in fluorescence enzyme-link immunoassay. The enzyme kinetic assay indicated that both the affinity and specificity of CPO to the substrate was improved in the buffer-ILS mixture, which was the main reason of the enhancement of product yield. This enzyme-catalyzed reaction was controlled mainly by the stability and consumption of CPO. The DAP yield was 81.16% under the optimum condition.

Key words: chloroperoxidase, σ-phenylenediamine, 2,3-diaminophenazine, ionic liquids, enzyme kinetic assay

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