Acta Chimica Sinica ›› 2002, Vol. 60 ›› Issue (11): 2029-2034. Previous Articles     Next Articles

Original Articles

高灵敏度纳喷雾正交飞行时间质谱法

余翀天;郭寅龙   

  1. 中国科学院上海有机化学研究所.上海(200032)
  • 发布日期:2002-11-15

High Sensitivity Nanoelectrospray Ionization Orthogonal Injection Time-of-flight Mass Spectrometry

Yu Chongtian;Guo Yinlong   

  1. Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences.Shanghai(200032)
  • Published:2002-11-15

A nanoelectrospray interface was set up for hyphenating with orthogonal injection time-of-flight mass spectrometer. Low detection limit (about 0.8 ~1.2 zmol) and low sample consumption (about 0.02 amol) per spectrum was obtained by injecting horse heart cytochrome c into the mass sepectrometer with a nanoelectrospray interface at a flow rate of 30 nL/min. As smaller droplet is formed in nanoelectrospray process, the desolvation efficiency and the ionization efficiency could be enhanced and thus may facilitate naalysis of oligosaccharides. A better signal was obtained in analyzing SBE-β-CD by the nanoelectrospray interface compared with a microspray interface. In addtion, in-source CID experiment of agiotensin I and trypsin enzymatic peptides map of cytochrome c were achieved by nanoelectrospray interface, respectively. The results showed that all but one ion in cytochrome c was achieved clearly in nanoelectrospray spectrum.

Key words: TIME-OFF FLIGHT MASS SPECTROMETRY, CYTOCHROME, POLYPEPTIDE

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