Determination of Hepatitis B Surface Antigen by Surface Plasma Resonance Biosensor

Acta Chimica Sinica ›› 2003, Vol. 61 ›› Issue (1): 137-140. Previous Articles Next Articles

Original Articles

表面等离子体子共振生物传感器用于乙肝表面抗原的测定

陈泽忠;王柯敏;羊小海;黄杉生;黄红梅;李杜;王青

湖南大学化学化工学院,长沙(410082)

发布日期:2003-01-15

Determination of Hepatitis B Surface Antigen by Surface Plasma Resonance Biosensor

Chen ZeZhong;Wang Kemin;Yang Xiaohai;Huang Shansheng;Huang Hongmei;Li Du;Wang Qing

Hunan UniversityState Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry & Chemical Engineering,Changsha (410082);Hunan University State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry & Chemical Engineering,Changsha (410082)

Published:2003-01-15

Abstract

A spectral surface plasmon resonance (SPR) biosensor has been used to determine the hepatitis B surface antigen (HB_sAg). The anti-HB_sAg monoclonal antibody was immobilized on the surface of the sensor chip using the technology of self-assembly and a bifunctional cross-linker. The detection limit of HB_sAg solution was 0.06ng/mL. The results of responses to HB_sAg control serum and to pure HB_sAg solution showed that the selectivity of SPR biosensor was good. The results of experiments showed that the detection limit of the SPR biosensor was much lower than that of enzyme-linked immuosorbent assay (ELISA) method.

Key words: DETERMINATION, PLASMA, RESONANCE, BIOSENSORS, SURFACES, ANTIGENS, HEPATITIS B

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Cite this article

Chen ZeZhong;Wang Kemin;Yang Xiaohai;Huang Shansheng;Huang Hongmei;Li Du;Wang Qing. Determination of Hepatitis B Surface Antigen by Surface Plasma Resonance Biosensor[J]. Acta Chimica Sinica, 2003, 61(1): 137-140.

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表面等离子体子共振生物传感器用于乙肝表面抗原的测定

Determination of Hepatitis B Surface Antigen by Surface Plasma Resonance Biosensor

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