Acta Chimica Sinica ›› 2003, Vol. 61 ›› Issue (3): 393-398. Previous Articles     Next Articles

Original Articles

中药材三七中皂苷类成分的近红外光谱快速无损分析新方法

杨南林;程翼宇;吴永江   

  1. 浙江大学化学工程与生物工程学系
  • 发布日期:2003-03-15

A New Method for Fast and Nondestructive Analysis of Saponins in Panax notoginseng Herb by Near Infrared Spectroscopy

Yang Nanlin;Cheng Yiyu;Wu Yongjiang   

  1. Department of Chemical & Biochemical Engineering,Zhejiang University
  • Published:2003-03-15

A new method using near infrared spectroscopy (NIRS) for fast and nondestructive determination of the active components in natural herb is proposed. HPLC was used as a reference method to determine the contents of notoginsenoside RI , ginsenoside Rgj, Ri"i, and Rd in Panax notoginseng herb, with colourimetry for Panax notoginseng saponins (PNS) . The contents of RI, Rg_1, Rb_1, Rd and PNS in the prepared 39 samples ranged in 1.58 -5.08, 21.68-46.13, 11.46-40.41, 3. 38-8.87, 64.22 - 145.13 mg-g~1 powder, respectively. Then, the near infrared diffuse reflectance spectra of the samples were acquired in 3500 - 11000 cm~l. Different wavenumber ranges and 11 spectrum preprocessing methods were investigated according to RMSECV values. Five calibration models were developed to correlate the spectra and the values determined by wet chemical analytical methods. The models were successfully applied to predict 8 unknown samples. RMSECV of the models for R_1 , Rg_1, Rb_1, Rd, PNS were 0.40, 1.47, 1.94, 0.53, 5.15 mg-g-1 herb, and RMSEP were 0.53, 3.15, 2.14, 0.70, 9.03 mg-g-1 herb, respectively. The method is fast, nondestructive and accurate. It provides a new efficient and environmental friendly method for determining the active components of natural herbs.

Key words: near infrared diffuse reflectance spectroscopy, CHINESE DRUG, ADSORPTION RESINS, SAPONINS

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