Acta Chimica Sinica ›› 2003, Vol. 61 ›› Issue (6): 885-888. Previous Articles     Next Articles

Original Articles

金属离子Cu~(2+),Co~(2+),Ni~(2+)的微芯片电泳分离及化学发光检测

苏荣国;林金明;屈锋;高云华;陈志锋   

  1. 中国科学院生态环境研究中心;中国科学院理化技术研究所
  • 发布日期:2003-06-15

Microchip Electropretic Separation of Cu~(2+), Co~(2+) and Ni~(2+) with Chemiluminescent Detection

Su Rongguo;Lin Jinming;Qu Feng;Gao Yunhua;Chen Zhifeng   

  1. Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences;Technical Institute of Physics and Chemistry, Chinese Academy of Sciences
  • Published:2003-06-15

A glass microchip was designed on the base of flow injection- chemiluminescent devices and fabricated by standard photolithgraphy technology, wet chemical etching and heat bonding technology. Electrophoresis separation was cooperated with chemiluminescent detection on the microchip, and the chemiluminescent reagents of luminol and hydrogen peroxide solution were delivered by a laboratory- made microfluidic pump. The experimental conditions including buffer applied, voltage and the flux of the chemiluminescent reagents were optimized. Under the optimal conditions, Cu~(2+) , Co~(2+) and Ni~(2+) were separated and detected with chemiluminescent method on the chip. The detection limits of Cu~(2+) , Co~(2+) and Ni~(2+) can reach 5. O*10~(-9), 5.O*10~(-11) and 1.0*10~(-7) mol/L, respectively.

Key words: FLOW INJECTION ANALYSIS, CHEMILUMINESCENCE ANALYSIS, LUMINOL, HYDROGEN PEROXIDE, COPPER ION, COBALT ION, NICKEL ION

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