The direct electrochem. of cytochrome c at microband gold electrode modified with a new promoter 4,6-dimethyl-2-mercaptopyrimidine was investigated. The self-assembly monolayer of 4,6-dimethyl-2-mercaptopyrimidine on the surface of gold electrode was characterized by IR spectrum and XPS. Cyclic voltammetric experiments indicated that the electrochem. of cytochrome c at gold electrode modified with this promoter was quasi-reversible with one-electron transfer reaction. The heterogeneous electron-transfer rate constant, ks, of cytochrome c was evaluated to be ca. 6.6?0-3cm/s. The effects of the immersion time and the oxygen in air on the promoter to promote the direct electrochem. of cytochrome c were discussed.

Key words: INFRARED SPECTROPHOTOMETRY, PHOTOELECTRON SPECTROSCOPY, ELECTROCHEMICAL REACTION, CHEMICAL MODIFIED ELECTRODE, ACCELERATORS, MONOMOLECULAR FILMS, CYCLOVOLTAMGRAPH