Electrochemical behavior,application and immobilization of glucose oxidase at nanometer-sized ultramicroband gold electrode

Acta Chimica Sinica ›› 1995, Vol. 53 ›› Issue (3): 281-285. Previous Articles Next Articles

Original Articles

纳米级微带金电极上葡萄糖氧化酶的固定.性质及应用

蔡称心;鞠煜先;陈洪渊

南京大学化学系

发布日期:1995-03-15

Electrochemical behavior,application and immobilization of glucose oxidase at nanometer-sized ultramicroband gold electrode

CAI CHENGXIN;JU YUXIAN;CHEN HONGYUAN

Published:1995-03-15

Abstract

The immobilization of glucose oxidase, as well as simultaneous immobilization of glucose oxidase and Fe(CN)63- as an electron mediator in polypyrrole film was successfully accomplished at a nanometer-sized ultramicroband gold electrode to provide a simple biosensor for detecting glucose without mediator in solution The GOD/PPy electrode was characterized by IR spectrum and cyclic voltammetry. The electropolymn. process of pyrrole and the effects of glucose oxidase on the electropolymn. pyrrole were also studied with chronoamperometry. The effects of polypyrrole thickness and oxygen in solution on the determination of glucose at GOD/Fe(CN)63-/PPy microelectrode were discussed.

Key words: FERRICYANIDE, INFRARED SPECTROPHOTOMETRY, MICROELECTRODE, CYCLOVOLTAMGRAPH, POLYPYRROLE, FIXED ENZYME, BIOSENSORS, GLUCOSE OXIDASE

CLC Number:

O646

Cite this article

CAI CHENGXIN;JU YUXIAN;CHEN HONGYUAN. Electrochemical behavior,application and immobilization of glucose oxidase at nanometer-sized ultramicroband gold electrode[J]. Acta Chimica Sinica, 1995, 53(3): 281-285.

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纳米级微带金电极上葡萄糖氧化酶的固定.性质及应用

Electrochemical behavior,application and immobilization of glucose oxidase at nanometer-sized ultramicroband gold electrode

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