Acta Chimica Sinica ›› 1994, Vol. 52 ›› Issue (11): 1112-1117. Previous Articles     Next Articles

Original Articles

二十种混合氨基酸的质谱鉴定 直接磷酰化物的正负离子FAB-MS分析

尹应武;麻远;赵玉芬;辛斌;王光辉   

  1. 清华大学化学系;中国科学院化学研究所
  • 发布日期:1994-11-15

The in situ positive and negative FAB-MS of the 20 amino acids phosphorylated by the dialkyphorphits

YIN YINGWU;MA YUAN;ZHAO YUFEN;XIN BIN;WANG GUANGHUI   

  • Published:1994-11-15

The pos. and neg. FAB-MS of the mixed twenty phosphorylated amino acids are reported. The triethylamine and glycerol matrix background was suppressed. By this in situ phosphorylation method, all twenty amino acids could be detected no matter how much phosphorylation reagent was used. The basic amino acids showed much greater response to the pos. FAB-MS. While the neutral and acidic amino acids were give much more intense mol. ion peaks in the neg. FAB-MS. This method could apply to the qual. detection of the amino acids in samples.

Key words: AMINO ACID, MASS SPECTROGRAPHY, PHOSPHORYLATION

CLC Number: