Acta Chimica Sinica ›› 1997, Vol. 55 ›› Issue (1): 76-82. Previous Articles     Next Articles

Original Articles

甲烷利用菌催化烯烃环氧化的底物选择性, 细胞失活原因及产物对映体组成

夏仕文;李树本;尉力;阮宗琴   

  1. 中国科学院兰州化学物理研究所;中国科学院羰基合成与选择氧化国家重点实验
  • 发布日期:1997-01-15

Substrate selectivity, cell deactivation, enantiomeric composition in epoxidation of alkenes catalyzed by methane-utilizing bacteria

XIA SHIWEN;LI SHUBEN;WEI LI;RUAN ZONGQIN   

  • Published:1997-01-15

Substrate selectivity, reasons for cell deactivation, enantiomeric composition of products in the epoxidation of alkenes catalyzed by methane-utilizing bacteria resting cell (Methylomonas sp. GYJ3, Methylomonas sp. S, Methylomonas sp. Z201, Methylosinus trichosporium IMV3011, Methylococcus capsulatus IMV3021) were studied. There were different epoxidizing activities for various bacterial strains and various substrates. Methane-utilizing bacteria were unable to epoxidize cyclohexene and srgrene, but short-chain alkenes. Activity of epoxidation was related to steric effect of allyl substrates XCH2CH=CH2(X=H, Cl, Br, OH). The main reasons for cell deactivation were cell-toxicity of products and lost of coenzyme NADH. Chiral capillary gas chromatography revealed that epoxidizing products of alkenes were racemic epoxides.

Key words: EPOXIDATION REACTION, ALKENE, ENANTIOMORPH, METHANE-UTILIZING BACTERIA

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