Dynamic interfacial tension of phospholipid /protem adsorption was studied by ADSA( axisymmetric drop shape analysis, DASA) to gain the information about the protein /phosphoilpid multilayer. AFM was used to study the morpholoigy of the ciomplex film. DPPE ( dipalmitoyl phosphatidylethanolamine ), DPPC( dipalnitoyl phosphatidylcholin), DPPA (dipalnitoyl phosphatidic acid) exhibitied quite different adsorption behavior in the step due to the net charge of their head group, but the interaction between phospholipid and protein seemed beyond the statistic force during the construction of protein /lipid multilayers when the different adsorption curves tended to coincide at the end. The adsorption process was dominated by different kind interaction at the different steps respectively.

Key words: PHOSPHOLIPID, LAMINATED FILMS, INTERACTIONS