Acta Chimica Sinica ›› 2014, Vol. 72 ›› Issue (3): 388-394.DOI: 10.6023/A13080911 Previous Articles     Next Articles

Special Issue: 石墨烯



张谦a,b, 吴抒遥a,b, 张玲c, 茆卉a,b, 刘大亮a,b, 刘洋d, 曾向群e, 宋溪明a,b, 李景虹d   

  1. a 辽宁大学绿色合成与先进材料制备化学辽宁省重点实验室 沈阳 110036;
    b 辽宁大学化学院 沈阳 110036;
    c 沈阳师范大学化学与生命科学学院 沈阳 110034;
    d 清华大学化学系 北京 100084;
    e 奥克兰大学化学系 罗切斯特市 美国
  • 投稿日期:2013-08-30 发布日期:2013-11-14
  • 通讯作者: 李景虹,;宋溪明,;
  • 基金资助:

    项目受国家自然科学基金(Nos. 21235004,21128005,51273087,21071070,21203126,21005046,51203072,20901035);辽宁省高等学校创新团队基金(No. LT2011001);辽宁省自然科学基金(Nos. 201202088,LJQ2013112);辽宁省博士科研启动基金项目(No. 20131042);辽宁大学青年科研基金(Nos. 2012LDQN08,2012LDQN07);辽宁大学创新创业训练计划项目(No. X201210140036)和辽宁大学创新人才培养基金资助.

Biocompatible Phospholipid Modified Graphene Nanocomposite for Direct Electrochemistry of Redox Enzyme

Zhang Qiana,b, Wu Shuyaoa,b, Zhang Lingc, Mao Huia,b, Liu Dalianga,b, Liu Yangd, Zeng Xiangqune, Song Ximinga,b, Li Jinghongd   

  1. a Liaoning Provincial Key Laboratory for Green Synthesis and Preparative Chemistry of Advanced Materials, Liaoning University, Shenyang 110036;
    b College of Chemistry, Liaoning University, Shenyang 110036;
    c College of Chemistry and Life Science, Shenyang Normal University, Shenyang 110034;
    d Department of Chemistry, Tsinghua University, Beijing 100084;
    e Department of Chemistry, Oakland University, Rochester USA
  • Received:2013-08-30 Published:2013-11-14
  • Supported by:

    Project supported by the National Natural Science Foundation of China (Nos. 21235004, 21128005, 51273087, 21071070, 21203126, 21005046, 51203072, 20901035), the Financial Supports from the Program for Liaoning Innovative Research Team in University (No. LT2011001), the Natural Science Foundation of Liaoning Province (Nos. 201202088, LJQ2013112), the Research Fund for the Doctoral Program of Liaoning Province (No. 20131042), the Foundation for Young Scholars of Liaoning University (Nos. 2012LDQN08, 2012LDQN07), College students' innovative entrepreneurial training program (No. X201210140036) and the Foundation of 211 Project for Innovative Talents Training, Liaoning University.

A novel lipid based carbonaceous nanocomposite, 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (sodium salt) (POPG) modified graphene (GP) (POPG-GP), was designed and synthesized by a non-covalent method. The nanocomposite was endowed with excellent properties of the two independent components, such as the biocompatibility of POPG and the outstanding electric properties of graphene. Fourier transform infrared (FT-IR) spectra, ultraviolet-visible (UV-vis) absorption spectra, transmission electron microscopy (TEM) were utilized to characterize the structure, morphology and surface property of the as synthesized POPG-GP. It has been found that the modification of POPG on GP could not only assist the dispersion of graphene in aqueous solution, but also endow it with negatively charged, which was favorable for the further immobilization of model enzyme via self-assembly. Based on the electrostatic interaction, the positively charged horseradish peroxide (HRP) could be immobilized onto the surface of POPG-GP to form HRP/POPG-GP/GC electrode. UV-vis and FT-IR spectroscopies were used to monitor the assembly process and demonstrated that HRP had been immobilized without denaturation. The HRP/POPG-GP/GC electrode could commendably realize the direct electron transfer (DET) between electrode and redox enzyme with good electrochemical performance. Moreover, such modified electrode also showed good electrocatalytic response toward the detection of H2O2 with high sensitivity, wide linear range, excellent stability and reproducibility. The linear response range for the HRP/POPG-GP/GC was 3.5~210 μmol/L (R=0.999). The detection limit and the sensitivity of the HRP/POPG-GP/GC electrode was calculated to be 1.17 μmol/L (S/N=3) and 356.6 mA·cm-2·M-1, respectively. The apparent Michaelis-Menten constant Km was estimated to be 0.45 mmol/L, indicating a high affinity of HRP to H2O2 on POPG-GP. The experiment results demonstrated that POPG-GP not only provided a biocompatible microenvironment for the immobilized HRP, but also supplied a necessary pathway for its direct electron transfer. Therefore, such biocompatible nanocomposite had potential applications in the field of biosensors.

Key words: graphene, phospholipid, nanocomposite, horseradish peroxide, direct electrochemistry