By both designing a novel electrolytic flow cell and using the flow- injection techniuqe, the nascent Br2 was in situ electrogenerated on the surface of platinum electrode with constant current electrolytic method in the H2SO4-KBr medium. It was then found that compared with reagent Br2 as well as on-line electrogenerated Br2, the in situ electrogenerated Br2 could oxidize the isoniazid injected accompanying by a stronger chemiluminescence signal. Based on this observation, a new flow-ijjection electrogenerated chemiluminescence method for the determination of isoniazid was proposed. At the same time, a new concept, which can be used to explore the CL properties of the in situ electrogenerated reagent and to improve the sensitivity of CL analysis, was also proposed.

Key words: NASCENCY, OXIDANT, BROMINE, ELECTROLYTIC CELL, FLOW INJECTION ANALYSIS, CHEMILUMINESSENCE, ELECTROLUMINESCENCE, ISONIAZIDUM