Acta Chimica Sinica ›› 2009, Vol. 67 ›› Issue (2): 167-173. Previous Articles     Next Articles

Original Articles

不稳定性心绞痛血瘀证的血浆蛋白质组学研究

赵慧辉 王 伟*

  

  1. (北京中医药大学研究生院 北京100029)
  • 投稿日期:2008-02-04 修回日期:2008-07-08 发布日期:2009-01-28
  • 通讯作者: 王伟

Plasma Proteomics Study on Unstable Angina Patients with Blood Stasis Syndrome

Zhao, Huihui Wang, Wei*   

  1. (Graduate School of Beijing University of Traditional Chinese Medicine, Beijing 100029)
  • Received:2008-02-04 Revised:2008-07-08 Published:2009-01-28
  • Contact: Wang, Wei

To seek differentially expressed plasma proteins of unstable angina patients with blood stasis syndrome, two-dimensional difference gel electrophoresis and mass spectrometry were used to screen the differentially expressed plasma proteins among such patients with blood stasis syndrome and healthy volunteers. Fibrinogen β chain, fibrinogen γ chain, α1-antitrypsin, haptoglobin β chain, haptoglobin α2 chain were significantly highly expressed in the plasma of these patients, while ApoA-IV, ApoA-I, transthyretin and ApoJ were decreased in their plasma. These identified increased expressed proteins could be divided into three categories according to their functions: (1) acute phase reactive protein, (2) apolipoprotein, (3) blood coagulation protein. Unstable angina with blood stasis syndrome may be correlated with inflammatory reaction, lipid metabolic disorder and blood coagulation factor abnormality, and these differentially expressed proteins could provide clues for the study of unstable angina biomarkers and discovery of new protein targets for antianginal drugs.

Key words: unstable angina, blood stasis syndrome, difference gel electrophoresis (DIGE), proteome, biomarker