A new molecularly imprinted polymer (MIP)-chemiluminescence (CL) detection approach towards recognition of dansyl-glycine (Gly) is presented. The polymer microspheres were synthesized using precipitation polymerization with glycine as template and immobilized in microtiter plates (96 wells) using poly(vinyl alcohol) (PVA) as glue. The analyte (dansyl-glycine) was selectively adsorbed on the MIP microspheres. After washing, the bound fraction was quantified based on a dansyl-glycine-bis- (2,4,6-trichlorophenyl) oxalate (TCPO)-H2O2 chemiluminescence reaction enhanced by imidazole. The chemiluminescence intensity has a linear relationship with the concentration of glycine in the range of 0.2～60 μmol/L, with the limit of detection (LOD) being 0.07 μmol/L (3σ). The relative standard deviation (RSD) for 11 parallel measurements of glycine (1 μmol/L) was 3.3%. The cavity of MIP synthesized with glycine as template is smaller, which can avoid non-specific adsorption and lead to enhancement of specificity, response speed and sensitivity when recognizing dansyl-glycine.

Key words: molecularly imprinted polymer, chemiluminescence, array sensor, glycine