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Acta Chimica Sinica ›› 2010, Vol. 68 ›› Issue (03): 257-262. Previous Articles Next Articles
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屈建莹*,陈文静
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Qu Jianying* Chen Wenjing
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A novel hydrogen peroxide biosensor was obtained by successfully immobilizing horseradish peroxidase (HRP) on a glassy carbon (GC) electrode, which was modified with multi-walled carbon nanotubes (MWNT) and alizarin red (AR). Then the biosensor was immerged in 3% glutaraldehyde (GA) in order to overcome the swelling and improve the stability of the gelatin membrane. The experimental condition was optimized, and the electrochemistry behavior of the HRP/AR/MWNT/GC biosensor in PBS (pH 7.0) and H2O2 was discussed. The biosensor displayed rapid response and expanded linear response range from 5.0×10-6 to 1.0×10-3 mol/L (R=0.9932) with detection limit of 1.0×10-7 mol/L in PBS (pH 7.0), as well as acceptable preparation reproducibility and excellent stability.
Key words: multi-walled carbon nanotube, alizarin red, gelatin, horseradish peroxidase, glutaraldehyde
JUE Jian-Ying, CHEN Wen-Jing. Hydrogen Peroxide Biosensor Based on Gelatin Immobilizing Horseradish Peroxidase Qu Jianying* Chen Wenjing[J]. Acta Chimica Sinica, 2010, 68(03): 257-262.
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