Fluorimetric Method for the Determination of Sequence-Specific DNA with the Fluorescent Dye Hoechst 33258

Acta Chimica Sinica ›› 2011, Vol. 69 ›› Issue (12): 1450-1456. Previous Articles Next Articles

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基于Hoechst33258荧光染料检测单链DNA的方法研究

曾国平^1,2，向东山²，何治柯^*,2

(¹湖北第二师范学院武汉 430205)
(²武汉大学化学与分子科学学院生物医学分析化学教育部重点实验室武汉 430072)

投稿日期:2010-09-26 修回日期:2010-12-29 发布日期:2011-02-25
通讯作者: 何治柯 E-mail:zhkhe@whu.edu.cn
基金资助:
国家自然科学基金项目

Fluorimetric Method for the Determination of Sequence-Specific DNA with the Fluorescent Dye Hoechst 33258

Zeng Guoping^1,2 Xiang Dongshan² He Zhike*^,2

(¹ Hubei University of Education, Wuhan 430205)
(²Key Laboratory of Analytical Chemistry for Biology and Medicine, Ministry of Education, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072)

Received:2010-09-26 Revised:2010-12-29 Published:2011-02-25

A fluorimetric method is developed for the sequence-specific DNA detection with the Hoechst, which displays no photoluminescence in the presence of ssDNA but enhances great in the presence of dsDNA. The interactions between Hoechst and ssDNA with different base sequence and different sequence length were investigated in detail. On the basis of that, the detection of sequence-specific DNA related to the avian influenza A H1N1 virus was established. Under the optimum conditions, the linear range is 5.6× 10^－10～3.0×10^－8mol/L, the calibration curve is y＝24.005x＋0.4858, R²＝0.9967. The detection limit of 4.8×10^－10mol/L could be estimated using 3σ (where σ was the relative standard deviation of a blank solution, n＝11). This method is simple, rapid, sensitive and selective.

Key words: fluorescent dye hoechst, sequence-specific DNA, hybrid, fluorescence spectra, quantitative detection

CLC Number:

中图分类号：O657　文献标识码：A

Cite this article

ZENG Guo-Ping, XIANG Dong-Shan, HE Zhi-Ke. Fluorimetric Method for the Determination of Sequence-Specific DNA with the Fluorescent Dye Hoechst 33258[J]. Acta Chimica Sinica, 2011, 69(12): 1450-1456.

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基于Hoechst33258荧光染料检测单链DNA的方法研究

Fluorimetric Method for the Determination of Sequence-Specific DNA with the Fluorescent Dye Hoechst 33258

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