In order to study the metabolism, mode of action and other environmental behaviors of novel fungicide pyraoxystrobin, a ¹⁴C labelled pyraoxystrobin was synthesized from 4-chlorobenzoic acid as ¹⁴C labeled carbon source via substitution, cyclization and condensation. Its chemical structure was identified by HPLC-MS (ESI), MS (EI) and ¹H NMR, its purity was determinated by HPLC with more than 98% in mass ratio. HPLC-LSC and TLC-IIA determination demonstrated that its radiochemical purity was more than 98% with specific activity of (5.042±0.076) mCi/mmol. According to the total synthetic yield, the total chemical yield and radiochemical yield were both 43.93%.

Key words: pyraoxystrobin, ¹⁴C labeled synthesis, identification, fungicide